Abstract

The aims of this project are to understand the biology of papillomaviruses as related to laryngeal papillomatosis. The four projects focus on basic cell-virus interactions, a possible new therapy for the disease, and studies of the immune system in patients in an attempt to understand their susceptibility to the recurring papillomas. Progress has been made in each of these areas. In the following year Project 1 will work on improving the cell culture conditions for normal differentiation of laryngeal cells, and using microinjection of Human Papillomavirus (HPV) DNA to study viral stability in different cells. Project 2 will test different ways of applying the photodynamic therapy (ie topical vs injected drug, topical vs implanted laser light) and confirm that this therapy does not carry any detectable risk of malignant conversion. Project 4 will study the replication of different HPV types in various types of cells in vitro. Project 5 will continue to study the cell mediated immune system in the patients.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS019214-05
Application #
3099740
Study Section
Communicative Disorders Review Committee (CDR)
Project Start
1983-08-01
Project End
1991-07-31
Budget Start
1987-08-01
Budget End
1988-07-31
Support Year
5
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Long Island Jewish Medical Center
Department
Type
DUNS #
City
New Hyde Park
State
NY
Country
United States
Zip Code
11040

  Publications

Abramson, A L; DiLorenzo, T P; Steinberg, B M (1990) Is papillomavirus detectable in the plume of laser-treated laryngeal papilloma? Arch Otolaryngol Head Neck Surg 116:604-7
Steinberg, B M; Meade, R; Kalinowski, S et al. (1990) Abnormal differentiation of human papillomavirus-induced laryngeal papillomas. Arch Otolaryngol Head Neck Surg 116:1167-71
DiLorenzo, T P; Steinberg, B M (1990) Laryngeal keratinocytes show variable inhibition of replication by TGF-beta. J Cell Sci 96 ( Pt 1):115-9
Brandwein, M; Steinberg, B; Thung, S et al. (1989) Human papillomavirus 6/11 and 16/18 in Schneiderian inverted papillomas. In situ hybridization with human papillomavirus RNA probes. Cancer 63:1708-13
Steinberg, B M; Auborn, K J; Brandsma, J L et al. (1989) Tissue site-specific enhancer function of the upstream regulatory region of human papillomavirus type 11 in cultured keratinocytes. J Virol 63:957-60
Di Lorenzo, T P; De Maro, J A; Pumo, D E (1989) Nutritional requirements of papillomavirus-transformed mouse cells and an uninfected parent line in serum-free culture. In Vitro Cell Dev Biol 25:909-13
Steinberg, B M; Gallagher, T; Stoler, M et al. (1988) Persistence and expression of human papillomavirus during interferon therapy. Arch Otolaryngol Head Neck Surg 114:27-32
Shikowitz, M J; Steinberg, B M; Galli, R L et al. (1988) Histological and molecular analysis of cottontail rabbit papillomavirus-induced papillomas treated with hematoporphyrin derivative photodynamic therapy. Arch Otolaryngol Head Neck Surg 114:175-8
Zaslav, A L; Steinberg, B M; Stamberg, J et al. (1988) Chromosomal analysis of recurrent laryngeal papillomas. Arch Otolaryngol Head Neck Surg 114:1170-4
Mullooly, V M; Abramson, A L; Steinberg, B M et al. (1988) Clinical effects of alpha-interferon dose variation on laryngeal papillomas. Laryngoscope 98:1324-9

Showing the most recent 10 out of 19 publications