Abstract

The purpose of this core is to make available various transgenic and knockout mutants for Projects by Giffard, Steinberg, and Chan. One particular strength of this Transgenic Animal Core is that Dr. Chan (Core Leader) will provide his expertise to the other projects in the use of superoxide dismutases, in both transgenic and knockout mutant mice, as well as oxygen radical-related biochemistry and molecular biology. In addition, Dr. Chan will provide his expertise in primary cell culture of neurons, astrocytes, and endothelial cells from rodents. This Core will serve as a centralized facility for set-up, characterization and quality control of these primary cell cultures from wild-type animals, as well as SOD1, SOD2, and other transgenic and knockout mutant mice. This Core will also provide expertise in the molecular analysis of gene expression (PCR, RT-PCR), gel electrophoresis, enzymatic activity of CuZnSOD and MnSOD and biochemical assays in mice and in primary cell cultures. In addition, the Core will be used to provide technical assistance in breeding the various transgenic and knockout mice for the various projects of this program. Thus, the Transgenic Animal Core provides a unique vehicle for the projects to achieve the research goals employing various transgenic and knockout animals.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Research Program Projects (P01)
Project #
5P01NS037520-07
Application #
7062927
Study Section
Special Emphasis Panel (ZNS1)
Project Start
Project End
Budget Start
2005-03-01
Budget End
2006-02-28
Support Year
7
Fiscal Year
2005
Total Cost
$199,952
Indirect Cost

  Institution

Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Knowland, Daniel; Arac, Ahmet; Sekiguchi, Kohei J et al. (2014) Stepwise recruitment of transcellular and paracellular pathways underlies blood-brain barrier breakdown in stroke. Neuron 82:603-17
Arac, Ahmet; Grimbaldeston, Michele A; Nepomuceno, Andrew R B et al. (2014) Evidence that meningeal mast cells can worsen stroke pathology in mice. Am J Pathol 184:2493-504
Daadi, Marcel M; Hu, Shijun; Klausner, Jill et al. (2013) Imaging neural stem cell graft-induced structural repair in stroke. Cell Transplant 22:881-92
Cheng, Michelle Y; Lee, Alex G; Culbertson, Collin et al. (2012) Prokineticin 2 is an endangering mediator of cerebral ischemic injury. Proc Natl Acad Sci U S A 109:5475-80
Encarnacion, Angelo; Horie, Nobutaka; Keren-Gill, Hadar et al. (2011) Long-term behavioral assessment of function in an experimental model for ischemic stroke. J Neurosci Methods 196:247-57
Horie, Nobutaka; Pereira, Marta P; Niizuma, Kuniyasu et al. (2011) Transplanted stem cell-secreted vascular endothelial growth factor effects poststroke recovery, inflammation, and vascular repair. Stem Cells 29:274-85
Andres, Robert H; Horie, Nobutaka; Slikker, William et al. (2011) Human neural stem cells enhance structural plasticity and axonal transport in the ischaemic brain. Brain 134:1777-89
Andres, Robert H; Choi, Raymond; Pendharkar, Arjun V et al. (2011) The CCR2/CCL2 interaction mediates the transendothelial recruitment of intravascularly delivered neural stem cells to the ischemic brain. Stroke 42:2923-31
Cheng, Michelle Y; Lee, I-Ping; Jin, Michael et al. (2011) An insult-inducible vector system activated by hypoxia and oxidative stress for neuronal gene therapy. Transl Stroke Res 2:92-100
Arac, Ahmet; Brownell, Sara E; Rothbard, Jonathan B et al. (2011) Systemic augmentation of alphaB-crystallin provides therapeutic benefit twelve hours post-stroke onset via immune modulation. Proc Natl Acad Sci U S A 108:13287-92

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