Abstract

The Pittsburgh Center Institute proposes to organize a SPORE for lung cancer (PCI-LCS) with the overall objective of devising effective strategies for preventing the development of lung cancer and for the treatment of patients after diagnosis of this very common and malignant disease. The proposed research projects are closely interconnected and involve a wide array of basic and clinical expertise, that is being focused on lung cancer. Projects are directed towards elucidating the molecular pathogenesis of this disease and identifying and evaluating biological markers that help to define high risk. Project 2 is focused on a detailed assessment of the whole body and local pulmonary activity of the isoenzymes of the cytochrome P450 system, to understand the genetic heterogeneity in susceptibility to environmental carcinogens and to identify additional biomarkers for risk of lung cancer. Project 3 is an epidemiologic study of heavy smokers at high risk of lung cancer, with a focus on assessment of serum oncoproteins as risk factors. Project 4 is a closely linked case control study of some of the high risk individuals, involving bronchoscopy to obtain HBE for detailed studies of differentiation markers and of P450 enzyme profiles.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Exploratory Grants (P20)
Project #
5P20CA058235-03
Application #
2098937
Study Section
Special Emphasis Panel (SRC (52))
Project Start
1992-09-30
Project End
1995-08-31
Budget Start
1994-09-01
Budget End
1995-08-31
Support Year
3
Fiscal Year
1994
Total Cost
Indirect Cost

  Institution

Name
University of Pittsburgh
Department
Type
Schools of Medicine
DUNS #
053785812
City
Pittsburgh
State
PA
Country
United States
Zip Code
15213

  Publications

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Zhang, H; Yousem, S A; Franklin, W A et al. (1998) Differentiation and programmed cell death-related intermediate biomarkers for the development of non-small cell lung cancer: a pilot study. Hum Pathol 29:965-71
Shriver, S P; Shriver, M D; Tirpak, D L et al. (1998) Trinucleotide repeat length variation in the human ribosomal protein L14 gene (RPL14): localization to 3p21.3 and loss of heterozygosity in lung and oral cancers. Mutat Res 406:9-23
Keohavong, P; Zhu, D; Melacrinos, A C et al. (1997) Detection of low-fraction K-ras mutations in primary lung tumors using a sensitive method. Int J Cancer 74:162-70
Siegfried, J M; Weissfeld, L A; Singh-Kaw, P et al. (1997) Association of immunoreactive hepatocyte growth factor with poor survival in resectable non-small cell lung cancer. Cancer Res 57:433-9
Keohavong, P; Zhu, D; Whiteside, T L et al. (1997) Detection of infrequent and multiple K-ras mutations in human tumors and tumor-adjacent tissues. Anal Biochem 247:394-403
Zhu, D; Zhou, J; Keohavong, P (1997) Taq-amplified fragments appear as doublets in denaturing gradient gels. Anal Biochem 244:404-6
Kasprzyk, P G; Sullivan, T L; Hunt, J D et al. (1996) Activity of anti-erbB-2 recombinant toxin OLX-209 on lung cancer cell lines in the absence of erbB-2 gene amplification. Clin Cancer Res 2:75-80
Weissfeld, J L; Larsen, R D; Niman, H L et al. (1996) Oncogene-related serum proteins and cancer risk: a nested case-control study. Am J Epidemiol 144:723-7
Romkes-Sparks, M; Mnuskin, A; Chern, H D et al. (1994) Correlation of polymorphic expression of CYP2D6 mRNA in bladder mucosa and tumor tissue to in vivo debrisoquine hydroxylase activity. Carcinogenesis 15:1955-61