This application to establish an IR-BU Planning Center is based on the explicit assumption that more widespread clinical applications of regenerative medicine technologies are contingent on an improved understanding of the organ regeneration and repair process in relevant model systems. To this end, we have assembled an interdisciplinary team that is uniquely qualified to pursue the focused aims of this Planning Center. The key personnel on the Planning Center team reflect an ideal union of investigators with extensive and complementary experience in research and clinical translation in the lower urinary tract, with recognized expertise in stem cell etiology), contribution and composition of tumor formation, as well as the role of stem cells and gene therapy in liver regeneration and hematopoiesis. An Educational Enrichment Program is proposed to increase institutional, national and international recognition of our efforts, and moreover, to provide an excellent environment and resources for training the next generation of translational urologic researchers. The scientific project specifically targets the estimated 35 million Americans that suffer from bladder disease, most of which are chronic conditions (i.e., infections, congenital disorders, and inflammation) that ultimately compromise cellular and tissue integrity leading to reduced bladder function, as well as a diminished quality of life for millions of patients. Despite our encouraging advancements in functional bladder regeneration in rats, it remains obvious that development of additional animal models is necessary to accelerate the pace of scientific progress toward clinical translation. To this end we propose the use of multicolored transplant procedures that will, for the first time, allow the identification of what cells or tissue is responsible for the badder repair/regeneration processes. Thus, we will incorporate novel imaging technology into our ongoing studies of functional bladder regeneration to provide an interdisciplinary approach to the study of bladder wall regeneration and repair as follows:
Specific Aim #1 : Evaluate and quantify the spatial and temporal characteristics of the cellular proliferation that mediates functional bladder regeneration in the mouse model.
Specific Aim #2 : Determine the contribution of local stem/progenitor cells versus bone marrow-derived stem/progenitor cells.

Public Health Relevance

Congenital deformities, infection, inflammation, traumatic injury, cancer and nerve lesions produce irreversible damage to bladder tissue resulting in removal and/or permanent impairment in bladder function. These conditions affect millions of Americans and lead to a poor quality of life. We will study the process of bladder regeneration in a relevant rodent model to better apply tissue engineering and regenerative medicine technologies to the creation of new functional tissue derived from the patients'own cells.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Exploratory Grants (P20)
Project #
5P20DK097806-02
Application #
8549233
Study Section
Special Emphasis Panel (ZDK1-GRB-6 (O3))
Program Officer
Hoshizaki, Deborah K
Project Start
2012-09-29
Project End
2014-07-31
Budget Start
2013-08-01
Budget End
2014-07-31
Support Year
2
Fiscal Year
2013
Total Cost
$296,000
Indirect Cost
$96,000
Name
Wake Forest University Health Sciences
Department
Internal Medicine/Medicine
Type
Schools of Medicine
DUNS #
937727907
City
Winston-Salem
State
NC
Country
United States
Zip Code
27157
Zarifpour, Mona; Andersson, Karl-Erik; Kelkar, Sneha S et al. (2017) Characterization of a Murine Model of Bioequivalent Bladder Wound Healing and Repair Following Subtotal Cystectomy. Biores Open Access 6:35-45
Stuart, Christopher H; Riley, Kathryn R; Boyacioglu, Olcay et al. (2016) Selection of a Novel Aptamer Against Vitronectin Using Capillary Electrophoresis and Next Generation Sequencing. Mol Ther Nucleic Acids 5:e386
Ross, Christina L; Siriwardane, Mevan; Almeida-Porada, Graça et al. (2015) The effect of low-frequency electromagnetic field on human bone marrow stem/progenitor cell differentiation. Stem Cell Res 15:96-108
Christ, George J; Saul, Justin M; Furth, Mark E et al. (2013) The pharmacology of regenerative medicine. Pharmacol Rev 65:1091-133