The present proposal is designed to obtain a complete physical and genetic map of human chromosome 13 (CHR13) within 5 years. The physical map will locate macro-restriction sites (NotI) at spacings of 1 mb or less and STS's at spacings of 0.1 mb or less, and will provide complete sets of YAC contigs and P1 clones, positioned on the macro-restriction map. The STSs will be derived from NotI linking clones, the ends of the YACs and P1 clones, and CHR13- specific anonymous cDNAs. A complete CHR13 genetic linkage map, at spacings of 2 cM or less, will be generated by CHR13 library screening, search for polymorphisms, typing of reference pedigrees and statistical analyses. To complete the map, intact and deleted CHR13 hybrids (rodent:human cell lines) will be used to map NotI linking and P1 clones, to bands and sub-bands of CHR13. The superimposed maps (macro- restriction and genetic linkage maps, contigs and assignment of probes to chromosomal bands and sub-bands) will be entered into a database (locally and into the national network) to provide access to both the data and the products (NotI linking, cDNA, P1 and YAC clones, and CHR13-specific human:rodent cell lines), to all interested investigators.

Agency
National Institute of Health (NIH)
Institute
National Human Genome Research Institute (NHGRI)
Type
Exploratory Grants (P20)
Project #
1P20HG000424-01
Application #
3100680
Study Section
Genome Research Review Committee (GRRC)
Project Start
1991-04-01
Project End
1993-03-31
Budget Start
1991-04-01
Budget End
1992-03-31
Support Year
1
Fiscal Year
1991
Total Cost
Indirect Cost
Name
Columbia University (N.Y.)
Department
Type
Schools of Medicine
DUNS #
064931884
City
New York
State
NY
Country
United States
Zip Code
10027
Cayanis, E; Russo, J J; Kalachikov, S et al. (1998) High-resolution YAC-cosmid-STS map of human chromosome 13. Genomics 47:26-43
Fischer, S G; Cayanis, E; Russo, J J et al. (1994) Assembly of ordered contigs of cosmids selected with YACs of human chromosome 13. Genomics 21:525-37
Zhang, P; Schon, E A; Fischer, S G et al. (1994) An algorithm based on graph theory for the assembly of contigs in physical mapping of DNA. Comput Appl Biosci 10:309-17
Weeks, D E; Lathrop, G M; Ott, J (1993) Multipoint mapping under genetic interference. Hum Hered 43:86-97
Petrukhin, K E; Speer, M C; Cayanis, E et al. (1993) A microsatellite genetic linkage map of human chromosome 13. Genomics 15:76-85
Brzustowicz, L M; Merette, C; Xie, X et al. (1993) Molecular and statistical approaches to the detection and correction of errors in genotype databases. Am J Hum Genet 53:1137-45
Petrukhin, K; Fischer, S G; Pirastu, M et al. (1993) Mapping, cloning and genetic characterization of the region containing the Wilson disease gene. Nat Genet 5:338-43
Warburton, D; Yu, M T; Tantravahi, U et al. (1993) Regional localization of 32 NotI-HindIII fragments from a human chromosome 13 library by a somatic cell hybrid panel and in situ hybridization. Genomics 16:355-60
Terwilliger, J D; Ding, Y; Ott, J (1992) On the relative importance of marker heterozygosity and intermarker distance in gene mapping. Genomics 13:951-6
Ott, J (1992) Strategies for characterizing highly polymorphic markers in human gene mapping. Am J Hum Genet 51:283-90