Answers to today's fundamental biomedical questions generally require the application of techniques and methodologies from multiple scientific disciplines. In this RIMI collaborative project, Drs. Macher and Kaplan will utilize a range of research tools and approaches, incmuding genetics, molecular biology, cell biology, and MS to identify and characterize proteins that have critical functions in the kinetochore complexes and the ceil cycle. Their preliminary results have demonstrated that Sgt1p forms transient interactions with the kinetochore complexes and that it is post transtationally modified by phosphorylation, in the proposed research, further studies will be executed to determine the importance of the phosphoryiation sites already identified, and using the new mass spectrometer (i.e. MALDI-TOF) that will be available through the proposed Protein Chemistry facility, determine if there are additional sites of phosphorylation, and their location in the Sgt1p sequence. Genetic experiments provide evidence that SGT1 regulates the transition of ceils through the yeast """"""""Start"""""""" point and the assembly of kinetochore complex. To determine whether SGT1 regulates these processes via a common pathway, or through multiple distinct pathways, we propose to analyze proteins associated with Sgt1p during the course of the cell cycle and under specific growth conditions: This will be accomplished by a combination of immunoprecipitation and proteomics (i.e. MS and database searching). The results of these studies will not only provide new insights into the regulation of the cell cycle and its relationships with kinetochore complex assembly and disassembly, but also identify potential targets for cancer drug design. As importantly, students from SFSU will gain valuable experience in a range of research topics and techniques that otherwise would not be available without this collaboration.
| Galli, Lisa M; Santana, Frederick; Apollon, Chantilly et al. (2018) Direct visualization of the Wntless-induced redistribution of WNT1 in developing chick embryos. Dev Biol 439:53-64 |
| Forma, Vincent; Anderson, David I; Goffinet, François et al. (2018) Effect of optic flows on newborn crawling. Dev Psychobiol 60:497-510 |
| Anderson, David I; Kobayashi, Yuka; Hamel, Kate et al. (2016) Effects of support surface and optic flow on step-like movements in pre-crawling and crawling infants. Infant Behav Dev 42:104-10 |
| Galli, Lisa M; Zebarjadi, Navid; Li, Lydia et al. (2016) Divergent effects of Porcupine and Wntless on WNT1 trafficking, secretion, and signaling. Exp Cell Res 347:171-83 |
| Galli, Lisa M; Barnes, Tiffany; Burrus, Laura W (2016) The Use of Chick Embryos to Study Wnt Activity Gradients. Methods Mol Biol 1481:69-80 |
| Barbu-Roth, Marianne; Anderson, David I; Streeter, Ryan J et al. (2015) Why does infant stepping disappear and can it be stimulated by optic flow? Child Dev 86:441-55 |
| Miranda, M; Galli, L M; Enriquez, M et al. (2014) Identification of the WNT1 residues required for palmitoylation by Porcupine. FEBS Lett 588:4815-24 |
| Galli, Lisa M; Munji, Roeben N; Chapman, Susan C et al. (2014) Frizzled10 mediates WNT1 and WNT3A signaling in the dorsal spinal cord of the developing chick embryo. Dev Dyn 243:833-843 |
| Galli, Lisa M; Szabo, Linda A; Li, Lydia et al. (2014) Concentration-dependent effects of WNTLESS on WNT1/3A signaling. Dev Dyn 243:1095-105 |
| Barbu-Roth, Marianne; Anderson, David I; Desprès, Adeline et al. (2014) Air stepping in response to optic flows that move Toward and Away from the neonate. Dev Psychobiol 56:1142-9 |
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