This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.VGN Lead Institution HighlightsContributed Towards Purchase of Mass Spectrometer for Bryan Ballif- Thermo Electron Corp LTQ02 10000 LTQ XL MS System- HP Laser Jet Printer 2420D Printer, 110VPurchased Speed Vac Concentrator for Proteomics Facility- Fisher Scientific Speed Vac Concentrator Basic- Fisher Scientific REF Vapor Trap 050C 4L- Fisher Scientific Vacuum Pump- Fisher Scientific 24X 1.5ML Rotor Pk 120VPurchased GeneSifter Software System for Bioinformatics Microarray Analysis- GeneSifter Microarray Analysis System Core EditionSeven Graduate Student Assistantships granted in Year 2 Four Graduate Student Assistantships granted in Year 3UVM DNA Microarray Facility: Activities/Highlights Services Offered:-Consultation on experimental design in collaboration with the Bioinformatics Core Facility-Gene Expression Profiling -target preparation -eukaryotic and prokaryotic -single and double amplification -Nugen based target preps for low RNA targets, i.e., LCM , cell sorting -Hybridization and scanning-DNA Mapping target preparation-Exon Array target preparation-RNA Integrity assessment-Nucleic Acid quantitation-RNA extraction assistance and training-SOP's established for all services offeredScientific or technical changes:-Optimized methods to provide exon array analysis -Optimized methods and developed protocols to recover intact RNA from fluorescence- activated cell sorting -Collaborating with the Bioinformatics Core to streamline online ordering of services, data dissemination, and data archiving-Development and optimization of methods for enrichment of intact DNA from FFPE samples for whole genome array analysis Professional Development: Staff attended and participated at the following meetings:-Tim Hunter and Scott Tighe, Annual Affymetrix Users Meeting, San Francisco, CA, May, 2006-Tim Hunter and Scott Tighe: Advances in Microarray Technology, October, 2006, Amsterdam, NL-Scott Tighe: Neuroblastoma Symposium, March 2007, Burlington, VT-Tim Hunter: Association of Biomolecular Resource Facilities, March 2007 Tampa, FLFacility Promotion: -Hosted Open House, June 16, 2006. Over 140 attendees -Provided Workshop on sample requirements and handling for a successful array experiment -Hosted Seminar: 'The Challenges and Successes of Implementing High Throughput Genomic and Proteomic Services in a Share Resource Facility' June, 2006, George Grills, Director of Core Operations and Technology Development, Cornell University-Hosted Technology Seminar: 'Utilizing miRNA in a Biological Study: A Process Overview', October 4, 2006, Rachele Kardon, Ambion Specialist-Hosted Technology Seminar, PCR Arrays: Focus on your Pathway with qRT-PCR', Jan. 17, 2007, Dr. Tim Steppe, Application Specialist, SuperArray.Facility Presentations:Invited Speaker:-Tim Hunter, Cambridge Health Institute, Oct. 2007, Providence, RI, 'Development of a Real-Time Quantitative PCR Assay: Choices and Strategies'-Tim Hunter, Northeast Regional Life Sciences Core Directors, Nov., 2007, Ithaca, NY, 'Management of Core Facilities' -Scott Tighe, Northeast Regional Life Sciences Core Directors, Nov., 2007, Ithaca, NY, Moderated roundtable discussion on 'Flow Cytometry: Cell Sorting for Downstream Transcriptional Analysis' -Tim Hunter, Association of Biomolecular Research Facilities, March, 2007, Tampa, FL. Moderated roundtable discussion on 'Gene Expression Troubleshooting'-Tim Hunter, University of Vermont OB/GYN Fellow Seminar Series, March, 2007 'UVM DNA and Microarray Facilities: What We Can Do For You'-Scott Tighe, University of Vermont, pre-meeting for VCC Neuroblastoma Symposium, Burlington, VT 'Overview of Microarray Technology and Applications' Meeting presentations (Poster): -Scott Tighe and Tim Hunter, Northeast Regional Life Sciences Core Directors, Nov., 2007, Ithaca, NY, Vermont Genetics Network Microarray FacilityOutcomes:Publications:Pulver-Kaste, RA, Barlow, CA, Penar, PL, Tranmer, B, and Lounsbury, KM. Ca2+-Dependent Transcription Patterns in Human Cerebrovascular Smooth Muscle. AM. J. Phys. Heart and Circ. Phys, H97-H105, 2006Muthusamy, V, Duraisamy, S, Bradbury, M, Hobbs, C, Curley, DP, Nelson, B, and Bosenberg, M. Epigenetic Silencing of Novel Tumor Supressors in Malignant Melanoma, Cancer Research; 66: (23), 11187-11193, 2006Grants received using DNA array data:Karen Lounsbury, HL67351-06 NIH, NHLBI Title: Calcium Signaling and Gene Expression in Vascular Smooth Muscle Cells, 2007-2012Utilization:-Experimental design consultations with 19 investigators-Completion of 20 microarray projects (237 GeneChips)-Performed 93 RNA assessments: 28 PicoChips and 65 NanoChips
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