This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The hypothesis underlying this project is that functionally important regulatory sequences will be conserved in the 5'- and 3'-non-coding flanking regions of orthologous genes from distantly related organisms such as mammals and elasmobranchs such that a comparative analysis of these regions will uncover potentially novel cis-acting transcriptional regulatory sequences. The first specific aim of the project is to clone cDNAs encoding related shark and skate growth factor receptors that have mammalian orthologues as a prelude to identifying upstream regulatory regions. We are cloning and sequencing 5'- and 3'-RACE PCR products generated with specific primers from shark and skate cDNAs derived from rectal gland, liver and skate embryos. We have sequenced from 1,000 to approximately 3,000 base pairs of four receptor kinase cDNAs from both shark and skate. Three of the cDNAs encode members of the fibroblast growth factor family of receptors. For all eight of the cDNAs we have partial 3'-non-coding region sequences, which we are trying to extend by 3'-RACE PCR.
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