This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This core focuses on identifying the nature of cells in skin and skeletal muscle after marrow cell transplantation. Donor cells and their specific lineages will be ascertained and colocialized by confocal and deconvolution fluorescent microscopy. Donor cells will be tracked in tissue sections or cell suspensions by expression of GFP, beta-galactosidase or the presence of make DNA. The lineage of identified donor cells will be ascertained by standard morphology, geographical location and immunohistochemsitry. Colocalization of donor and lineage markers will be confirmed using confocal or deconbolution fluorescent microscopy. The core will provide high-level expertise for these approaches. The core contains two Zeiss fluorescent motor-driven microscopes, an upright Axioplan 2 and an inversted Axiovert 200M, and a Zeiss 510 confocal laser-scanning microscope capable of four-color imaging. The core will be capable of standard deconvolution fluorescent microscopy, 3D reconstructions and confocal microscopy. These latter, plus photomapping are used to simultaneously show a donor marker and a cell type specific marker. The core is open to COBRE participants and there are no charges.
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