This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The Transgenic, Gene Targeting and Genotyping Core integrates with and enhances the capabilities of our existing Transgenic and Gene Targeting Facility with a proven track record of success in creating transgenic and knockout mice. This core will assist COBRE investigators by providing cost-effective services for thegeneration, characterization, and maintenance of genetic mouse models. Over one third of the members of our center (8/20) and 4 of the 5 COBRE projects for new investigators will utilize this facility. This facility will provide all COBRE scientists with the service to microinject DNA into fertilized eggs and transfer embryos to recipient mothers to create transgenic mice. In addition, the facility will provide services to create chimeric mice, including the maintenance of several ES cell lines, electroporation and selection of ES cells, karyotype determination and blastocyst injection of selected ES cells. COBRE funds will be used to expand these services to include the manipulation and preparation of DNA for microinjection or gene targeting, and genotyping of transgenic and knockout mice. The facility also provides cryopreservation of mouse lines. The COBRE funds will subsidize services for the junior investigators and center members and the institution will continue to provide significant state support for this facility. The facility is staffed by a director and two research associates who are experts with the method required and who will interface with two COBRE coadvisors.
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