The Skin Phenotyping Core of the Skin Disease Research Center provides a wide array of histological, immunohistochemical, and molecular-based tools to characterize gross and microscopic morphology, functional features and gene expression in skin of mutant and genetically engineered laboratory animals. Special technical expertise within this core includes extensive knowledge of skin and hair follicle morphology essential for understanding the genotype/phenotype correlations;state of the art methods for gene expression studies at both mRNA and protein level specifically designed for mouse and human skin;and possession of a battery of original experimental approaches to the study of skin physiology in vivo. The Core will assist Center investigators as well as other researchers at Columbia in evaluating the significance of a phenotypic manifestation or unanticipated skin effects of a genetic mutation, drug, or other experimental manipulation;to understand the possible functions of genes and molecular pathways implicated in skin/hair phenotype development and to analyze skin expression patterns of genes of investigator's interest at the mRNA and protein level using immunohistochemistry and in situ hybridization. In addition, the Core will provide investigators with proof reading of and suggestions for Materials &Methods sections for publications and applications for extramural research funding utilizing skin phenotype analysis. The Skin Phenotyping Core also serves as a center of communication between investigators providing the ground for exchange of samples, antibodies, techniques and ideas. The Core brings investigators working in similar projects and techniques together thus reducing the costs and increasing efficiency of research.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR044535-09
Application #
8121512
Study Section
Special Emphasis Panel (ZAR1)
Project Start
Project End
Budget Start
2010-07-01
Budget End
2011-06-30
Support Year
9
Fiscal Year
2010
Total Cost
$141,931
Indirect Cost
Name
Columbia University (N.Y.)
Department
Type
DUNS #
621889815
City
New York
State
NY
Country
United States
Zip Code
10032
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Mathew, Grinu; Hannan, Abdul; Hertzler-Schaefer, Kristina et al. (2016) Targeting of Ras-mediated FGF signaling suppresses Pten-deficient skin tumor. Proc Natl Acad Sci U S A 113:13156-13161
Shen, Yao; Kim, Arianna L; Du, Rong et al. (2016) Transcriptome Analysis Identifies the Dysregulation of Ultraviolet Target Genes in Human Skin Cancers. PLoS One 11:e0163054
Dai, Zhenpeng; Xing, Luzhou; Cerise, Jane et al. (2016) CXCR3 Blockade Inhibits T Cell Migration into the Skin and Prevents Development of Alopecia Areata. J Immunol 197:1089-99
Abaci, Hasan E; Guo, Zongyou; Coffman, Abigail et al. (2016) Human Skin Constructs with Spatially Controlled Vasculature Using Primary and iPSC-Derived Endothelial Cells. Adv Healthc Mater 5:1800-7
Dainichi, Teruki; Hayden, Matthew S; Park, Sung-Gyoo et al. (2016) PDK1 Is a Regulator of Epidermal Differentiation that Activates and Organizes Asymmetric Cell Division. Cell Rep 15:1615-23
Johnson, Dylan; Mathur, Mohit C; Kobayashi, Tomoyoshi et al. (2016) The Cardiomyopathy Mutation, R146G Troponin I, Stabilizes the Intermediate ""C"" State of Regulated Actin under High- and Low-Free Ca(2+) Conditions. Biochemistry 55:4533-40
Shinkuma, Satoru; Guo, Zongyou; Christiano, Angela M (2016) Site-specific genome editing for correction of induced pluripotent stem cells derived from dominant dystrophic epidermolysis bullosa. Proc Natl Acad Sci U S A 113:5676-81
Kim, Arianna L; Back, Jung Ho; Zhu, Yucui et al. (2016) AKT1 Activation is Obligatory for Spontaneous BCC Tumor Growth in a Murine Model that Mimics Some Features of Basal Cell Nevus Syndrome. Cancer Prev Res (Phila) 9:794-802
Sun, Xiaoyun; Kim, Arianna; Nakatani, Masashi et al. (2016) Distinctive molecular responses to ultraviolet radiation between keratinocytes and melanocytes. Exp Dermatol 25:708-13

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