Although standard techniques are available, intra-laboratory variation in the quality andnumber of bone cells available for research purposes at Yale has varied considerably. Toaddress this limitation and to advance productive scholarship, the Yale Core Center forMusculoskeletal Disorders developed the Cell Core. The mission of the Cell Core is tofacilitate the experimental use of authentic, primary, mouse and rat osteoblastic cells as wellas osteoclasts generated from in vitro culture.As part of our new initiatives bone marrow derived mesenchymal stem cells (MSCs) are nowavailable to Core investigators. Although outside the original mandate of the Core, theDirector has made his PIXImus machine available to Center members through an agreementwith the Densitometry subunit of the Physiology Core.
The Specific Aims of the current proposal are to provide the following services:1. Isolate and cultivate primary murine and fetal rat calvarial osteoblasts. This will includelarge-scale preparations for biochemical studies and RNA and DNA purification.2. Cultivate murine osteoclast-like cells generated from: 1) in vitro co-culture 2) culture with MCSFand RANKL.3. Maintain a wide range of frozen cell lines.4. Provide small animal bone densitometry (DEXA).5. Cultivate mouse bone marrow derived mesenchymal stem cells (MSCs).6. Train for in vitro isolation and culture of osteoblasts, osteoclasts and stromal cells, so thatinvestigators, appropriately trained, can initiate and maintain these cells in their laboratories.These services are coordinated under the directorship of Dr. Mark Horowitz, who has hadextensive experience with cell culture in general and the isolation and culture of bone cellsspecifically. The Cell Core is particularly anxious to support current and future Pilot andFeasibility Projects as part of the Center's ongoing effort to attract new investigators tomusculoskeletal research.PERFORMANCE SITE(S) (organization, ci
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