The Analytic and Preparative Flow Cytometry Core (APFCC) is designed to enhance the power andproductivity of the research of Rheumatic Disease Core Center (RDCC) investigators by providing state-ofthe-art flow cytometry for cell analyses and purification at a reasonable cost. To accomplish this goal, theCore provides the equipment, service and expertise necessary for the application of flow cytometry andrelated technologies to cutting edge research in the rheumatic diseases. We have used nationally peerreviewedfederal instrumentation grants and UAB resources to obtain top-of-the-line equipment that providesa wide range of applications for RDCC investigators. This includes a 20-detector, 4-laser analytic cytometer(obtained 2006), a 14-detector, 3-laser, a sort enhanced all digital sorting cytometer (upgraded 2006), a 6-detector, loader equipped analyzer, and a 5-detector analyzer. In addition, the core provides equipment forancillary techniques such as automated sample preparation (acquired 2005), spectrofluorimetry, andautomated magnetic enrichment (acquired 2005). We have developed new techniques as well as providedbasic sample analysis, with the result that the APFCC has served over 120 independently funded UABinvestigators and contributed to many publications. Our goals for the core are to continue efforts at outreachand education, to alert RDCC investigators to the potential for flow technology to increase the power of theirresearch, service, to improve efficiency and to maintain the highest possible standards of quality, andapplication development, to continue to bring new technologies and protocols to the RDCC researchcommunity. To achieve these goals, funds are requested to support outreach, educational, quality controland development activities of the Core Director and Core Manager and partially offset the costs of oneservice contract to maintain uninterrupted access to this vital technology. The-Scientific Advisory Committee,which combines both knowledgeable users and those involved in senior research positions at UAB, anexternal advisory, who is well informed about newer equipment and applications, and an integration with thelarger UAB institution will provide feedback and drive further development. With these efforts, we willmaintain flow cytometry as a fundamental, vital tool for state-of-the-art research in the rheumatic diseases atUAB.

Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Center Core Grants (P30)
Project #
5P30AR048311-07
Application #
7669289
Study Section
Special Emphasis Panel (ZAR1)
Project Start
2008-09-01
Project End
2012-08-31
Budget Start
2008-09-01
Budget End
2009-08-31
Support Year
7
Fiscal Year
2008
Total Cost
$119,940
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
DUNS #
063690705
City
Birmingham
State
AL
Country
United States
Zip Code
35294
Garner, Evan F; Williams, Adele P; Stafman, Laura L et al. (2018) FTY720 Decreases Tumorigenesis in Group 3 Medulloblastoma Patient-Derived Xenografts. Sci Rep 8:6913
Ladowski, Joseph M; Reyes, Luz M; Martens, Gregory R et al. (2018) Swine Leukocyte Antigen Class II Is a Xenoantigen. Transplantation 102:249-254
Harms, Ashley S; Thome, Aaron D; Yan, Zhaoqi et al. (2018) Peripheral monocyte entry is required for alpha-Synuclein induced inflammation and Neurodegeneration in a model of Parkinson disease. Exp Neurol 300:179-187
Lever, Jeremie M; Yang, Zhengqin; Boddu, Ravindra et al. (2018) Parabiosis reveals leukocyte dynamics in the kidney. Lab Invest 98:391-402
Chakraborty, Asmi; Dorsett, Kaitlyn A; Trummell, Hoa Q et al. (2018) ST6Gal-I sialyltransferase promotes chemoresistance in pancreatic ductal adenocarcinoma by abrogating gemcitabine-mediated DNA damage. J Biol Chem 293:984-994
Su, Hairui; Sun, Chiao-Wang; Liu, Szu-Mam et al. (2018) Defining the epigenetic status of blood cells using a cyanine-based fluorescent probe for PRMT1. Blood Adv 2:2829-2836
Shin, Boyoung; Kress, Robert L; Kramer, Philip A et al. (2018) Effector CD4 T cells with progenitor potential mediate chronic intestinal inflammation. J Exp Med 215:1803-1812
Gibson, Sara A; Yang, Wei; Yan, Zhaoqi et al. (2018) CK2 Controls Th17 and Regulatory T Cell Differentiation Through Inhibition of FoxO1. J Immunol 201:383-392
Stafman, Laura L; Mruthyunjayappa, Smitha; Waters, Alicia M et al. (2018) Targeting PIM kinase as a therapeutic strategy in human hepatoblastoma. Oncotarget 9:22665-22679
Jimenez, Rachel V; Wright, Tyler T; Jones, Nicholas R et al. (2018) C-Reactive Protein Impairs Dendritic Cell Development, Maturation, and Function: Implications for Peripheral Tolerance. Front Immunol 9:372

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