Form and function are intimately interrelated. However, the histological analyses of our target tissues (bone, cartilage, tendon, ligament, muscle) provide specialized challenges not often encountered when conducting these techniques on non-skeletal tissues. For example, due to the extensive extracellular matrix, these tissues can be very difficult to prepare and section, and bone presents a particular challenge due to mineralization. A specialized knowledge of the stages of development, and limb and joint morphology is necessary for the analysis of skeletal tissues. In addition to morphology, the localization of proteins and mRNA in mouse models of musculoskeletal diseases is a vital component in the analysis of the molecular mechanism of protein function, the response to injury, and disease. The overall objective of the In situ Molecular Analysis Core is to provide histological services for the identification and analysis of molecular phenotypes of our target tissues, bone, cartilage, muscle, tendon and ligament, in developing and adult mice. The services will include preparation of tissues and tissue sections;a reagent bank for histology, immunohistochemistry and in situ hybridization;and training in the techniques of in situ hybridization to mRNA, immunohistochemistry, deconvolution microscopy, and histomorphometry. Reagents for the analysis of mouse, and in some cases human, tissues will be available. We expect that the findings from mouse models will be extended to human disease and injury. The Director/Co-Directors and collaborating investigators will provide consultation and advice on analysis and interpretation of the target tissue phenotypes. Deconvolution microscopic analysis and bone histomorphometry will provide the opportunity to utilize sophisticated techniques and quantitative analysis not currently available to all investigators. The strength of this Core lies in the specific techniques developed for adult mice, sectioning delicate early healing tissue, and properly aligning blocks for sectioning of ligament and fracture healing, as well as the expertise in tissue staining by a wide variety of histochemical and molecular techniques. All aspects of the core provide collaboration, training, and education, which will strengthen ties among the research base and enhance the quality of the science performed. Thus, not only will this Core be cost-effective, it will allow scientists to explore skeletal phenotypes that they could not in any other way investigate. This core will complement the specific functional analyses proposed in Core B and the mouse models of Core D.

Public Health Relevance

The In Situ Molecular Analysis Core (C) will allow our scientists to maximize the amount of data derived from animal models of osteoporosis, arthritis, musculoskeletal injury, cancer metastasis to bone, and other musculoskeletal diseases by providing access to a number of sophisticated techniques and reagents.

National Institute of Health (NIH)
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Center Core Grants (P30)
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Special Emphasis Panel (ZAR1-CHW-G)
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Washington University
Saint Louis
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Murali, Bhavna; Ren, Qihao; Luo, Xianmin et al. (2018) Inhibition of the Stromal p38MAPK/MK2 Pathway Limits Breast Cancer Metastases and Chemotherapy-Induced Bone Loss. Cancer Res 78:5618-5630
Patra, Debabrata; DeLassus, Elizabeth; Mueller, Jennifer et al. (2018) Site-1 protease regulates skeletal stem cell population and osteogenic differentiation in mice. Biol Open 7:
Killian, Megan L; Locke, Ryan C; James, Michael G et al. (2018) Novel model for the induction of postnatal murine hip deformity. J Orthop Res :
Shen, Hua; Jayaram, Rohith; Yoneda, Susumu et al. (2018) The effect of adipose-derived stem cell sheets and CTGF on early flexor tendon healing in a canine model. Sci Rep 8:11078
Williams, Matthew J; Sugatani, Toshifumi; Agapova, Olga A et al. (2018) The activin receptor is stimulated in the skeleton, vasculature, heart, and kidney during chronic kidney disease. Kidney Int 93:147-158
Linderman, Stephen W; Shen, Hua; Yoneda, Susumu et al. (2018) Effect of connective tissue growth factor delivered via porous sutures on the proliferative stage of intrasynovial tendon repair. J Orthop Res 36:2052-2063
Guilak, Farshid; Nims, Robert J; Dicks, Amanda et al. (2018) Osteoarthritis as a disease of the cartilage pericellular matrix. Matrix Biol 71-72:40-50
Wang, Cuicui; Silverman, Richard M; Shen, Jie et al. (2018) Distinct metabolic programs induced by TGF-?1 and BMP2 in human articular chondrocytes with osteoarthritis. J Orthop Translat 12:66-73
McAndrew, Christopher M; Agarwalla, Avinesh; Abraham, Adam C et al. (2018) Local bone quality measurements correlates with maximum screw torque at the femoral diaphysis. Clin Biomech (Bristol, Avon) 52:95-99
Rohatgi, Nidhi; Zou, Wei; Collins, Patrick L et al. (2018) ASXL1 impairs osteoclast formation by epigenetic regulation of NFATc1. Blood Adv 2:2467-2477

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