Flow Cytometry Shared Resource

Grayson, Jason Mitchell

Abstract

The goal of the Flow Cytometry Shared Resource (FCSR) is to provide Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) members with ready access to the necessary expertise and state-of-the-art flow cytometry instrumentation that can be applied to a wide range of cancer biology research. Flow cytometry is a critical technology that provides rapid single-cell analysis and cell sorting through the simultaneous detection of light scatter properties and the presence of multiple cellular molecules or metabolites in or on an individual cell. In addition, the FCSR seeks to foster the development of protocols that can utilize the continually increasing number of available markers and methodologies. The FCSR is focused on addressing the needs of both seasoned flow cytometry users as well as those just discovering the capabilities of flow cytometry technology. To this end, monthly training for the instruments is provided for new users as well as support in experimental design and data interpretation following training. Instruments available include a BD FACS Aria cell sorter (three lasers and eleven parameters), a BD FACS Canto II analyzer (three lasers and ten parameters) and BD FACS Calibur and Accuri analyzers (two lasers and six parameters). In addition to the software with each flow cytometer, additional analysis software is available to analyze multiparameter data to identify and characterize cell subpopulations. DNA histogram analysis software programs are also available for researchers requiring advanced cell cycle analysis. The FCSR is under the direction of Martha Alexander-Miller, Ph.D., and has a staff of 1.33 FTEs. To accomplish the goals of the facility, the FCSR has the following Specific Aims to: 1) Provide cutting-edge analysis instrumentation for flow cytometric analysis; 2) Provide multi-parameter cell sorting for user-defined populations; 3) Provide instrument training for new and existing users; and 4) Provide expertise for experimental design and interpretation of flow cytometry data. In the November 2014 ? October 2015 grant year, the FCSR provided services to forty-two users, 74% of whom were WFBCCC members; of those, 64% had peer-reviewed funding. WFBCCC members accounted for 85% of the service hours.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Cancer Institute (NCI)
• Type: Center Core Grants (P30)
• Project #: 5P30CA012197-45
• Application #: 9848534
• Study Section: Subcommittee H - Clinical Groups (NCI)
• Budget Start: 2020-02-01
• Budget End: 2021-01-31
• Support Year: 45
• Fiscal Year: 2020

Institution

• Name: Wake Forest University Health Sciences
• DUNS #: 937727907
• City: Winston-Salem
• State: NC
• Country: United States
• Zip Code: 27157

Publications

Haas, Karen M; Johnson, Kristen L; Phipps, James P et al. (2018) CD22 Promotes B-1b Cell Responses to T Cell-Independent Type 2 Antigens. J Immunol 200:1671-1681

Park, Sun H; Keller, Evan T; Shiozawa, Yusuke (2018) Bone Marrow Microenvironment as a Regulator and Therapeutic Target for Prostate Cancer Bone Metastasis. Calcif Tissue Int 102:152-162

Suo, Xubin; Eldridge, Brittany N; Zhang, Han et al. (2018) P-Glycoprotein-Targeted Photothermal Therapy of Drug-Resistant Cancer Cells Using Antibody-Conjugated Carbon Nanotubes. ACS Appl Mater Interfaces 10:33464-33473

Widner, D Brooke; Park, Sun H; Eber, Matthew R et al. (2018) Interactions Between Disseminated Tumor Cells and Bone Marrow Stromal Cells Regulate Tumor Dormancy. Curr Osteoporos Rep 16:596-602

Liu, Liang; Ruiz, Jimmy; O'Neill, Stacey S et al. (2018) Favorable outcome of patients with lung adenocarcinoma harboring POLE mutations and expressing high PD-L1. Mol Cancer 17:81

Sirkisoon, Sherona R; Carpenter, Richard L; Rimkus, Tadas et al. (2018) Interaction between STAT3 and GLI1/tGLI1 oncogenic transcription factors promotes the aggressiveness of triple-negative breast cancers and HER2-enriched breast cancer. Oncogene 37:2502-2514

Goyal, Amrita; Carter, Joi B; Pashtan, Itai et al. (2018) Very low-dose versus standard dose radiation therapy for indolent primary cutaneous B-cell lymphomas: A retrospective study. J Am Acad Dermatol 78:408-410

Su, Weijun; Hong, Lixin; Xu, Xin et al. (2018) miR-30 disrupts senescence and promotes cancer by targeting both p16INK4A and DNA damage pathways. Oncogene 37:5618-5632

Miller Jr, David P; Denizard-Thompson, Nancy; Weaver, Kathryn E et al. (2018) Effect of a Digital Health Intervention on Receipt of Colorectal Cancer Screening in Vulnerable Patients: A Randomized Controlled Trial. Ann Intern Med 168:550-557

Bonin, Keith; Smelser, Amanda; Moreno, Naike Salvador et al. (2018) Structured illumination to spatially map chromatin motions. J Biomed Opt 23:1-8