The transgenic shared resource was founded in 1988 to train and assist AECC investigators in theproduction and characterization of transgenic mouse strains. This technology allows analysis of genefunction through the introduction of DMA sequences into the germ line, usually, although not exclusively, bypronuclear injection of fertilized oocytes. The shared resource supervisor director consults with individualinvestigators and advises on transgene construct, as well as making plasmids and sequence cassettesavailable, as well as other reagents. This results in DMA constructs suitable for expression in the mouse.Usually this is in the form of a plasmid but we also offer the injection of BAG DMA. The shared resourceprepares the DNA and introduces this DNA into the oocytes by micro-injection. In some cases the sequenceof interest is introduced into the oocyte by lentivirus infection. After the manipulation of the oocyte they aretransferred to pseudo-pregnant mothers and following birth, the pups are cared for to weaning. At this pointmice are returned to the investigator for further analysis. The production of useful transgenics usually takesless than seven weeks from submission of the DNA. We guarantee at least three founders per constructalthough generally more are generated. We have had essentially 100% success rate with little need for reinjectionof constructs, Between 18-25 investigators use this service per year with approximately 100constructs injected. In addition, the core provides embryo and sperm freezing for long-term storage of mouselines, in vitro fertilization (IVF) and intra-cytoplasmic sperm injections (ICSI) to rescue frozen samples ordifficult to mate strains. We also provide blastomere fusion to generate tetraploid embryos for aggregationwith ES cells to determine whether the phenotypes of mutations are intrinsic to the embryo or are the resultof placenta! abnormalities.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
2P30CA013330-35
Application #
7506872
Study Section
Subcommittee G - Education (NCI)
Project Start
2007-09-25
Project End
2012-06-30
Budget Start
2007-09-25
Budget End
2008-06-30
Support Year
35
Fiscal Year
2007
Total Cost
$185,730
Indirect Cost
Name
Albert Einstein College of Medicine
Department
Type
DUNS #
110521739
City
Bronx
State
NY
Country
United States
Zip Code
10461
Sharma, Yogeshwar; Liu, Jinghua; Kristian, Kathleen E et al. (2018) In Atp7b-/- Mice Modeling Wilson's Disease Liver Repopulation with Bone Marrowderived Myofibroblasts or Inflammatory Cells and not Hepatocytes is Deleterious. Gene Expr :
Iqbal, Niloy Jafar; Lu, Zhonglei; Liu, Shun Mei et al. (2018) Cyclin-dependent kinase 4 is a preclinical target for diet-induced obesity. JCI Insight 3:
De Martino, Daniela; Yilmaz, Emrullah; Orlacchio, Arturo et al. (2018) PI3K blockage synergizes with PLK1 inhibition preventing endoreduplication and enhancing apoptosis in anaplastic thyroid cancer. Cancer Lett 439:56-65
Norwood Toro, Laura E; Wang, Yarong; Condeelis, John S et al. (2018) Myosin-IIA heavy chain phosphorylation on S1943 regulates tumor metastasis. Exp Cell Res 370:273-282
Agalliu, Ilir; Chen, Zigui; Wang, Tao et al. (2018) Oral Alpha, Beta, and Gamma HPV Types and Risk of Incident Esophageal Cancer. Cancer Epidemiol Biomarkers Prev 27:1168-1175
Bhargava, Ragini; Sandhu, Manbir; Muk, Sanychen et al. (2018) C-NHEJ without indels is robust and requires synergistic function of distinct XLF domains. Nat Commun 9:2484
Collu, Giovanna M; Jenny, Andreas; Gaengel, Konstantin et al. (2018) Prickle is phosphorylated by Nemo and targeted for degradation to maintain Prickle/Spiny-legs isoform balance during planar cell polarity establishment. PLoS Genet 14:e1007391
Doyle, Christopher R; Moon, Jee-Young; Daily, Johanna P et al. (2018) A Capsular Polysaccharide-Specific Antibody Alters Streptococcus pneumoniae Gene Expression during Nasopharyngeal Colonization of Mice. Infect Immun 86:
Anayannis, Nicole V; Schlecht, Nicolas F; Ben-Dayan, Miriam et al. (2018) Association of an intact E2 gene with higher HPV viral load, higher viral oncogene expression, and improved clinical outcome in HPV16 positive head and neck squamous cell carcinoma. PLoS One 13:e0191581
Stepankova, Martina; Bartonkova, Iveta; Jiskrova, Eva et al. (2018) Methylindoles and Methoxyindoles are Agonists and Antagonists of Human Aryl Hydrocarbon Receptor. Mol Pharmacol 93:631-644

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