Abstract

The peptide synthesis facility contains an Applied Biosystems 432A Synergy peptide synthesizer, located in the Molecular Biology and Virology Laboratory. The instrument provides a convenient source of peptides that are used for preparing antibodies, identifying phosphorylation sites in proteins, identifying regions of protein-protein interactions, preparing affinity columns for protein isolation and purification, assaying protein kinase and phosphatase activities and studying the specificity of enzyme- substrate interactions, as well as peptides that are used in biological assays either synthetically fused to amino acid sequences with membrane permeation properties or covalently coupled to carrier proteins for microinjection.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA014195-27
Application #
6300011
Study Section
Project Start
1999-12-01
Project End
2000-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
27
Fiscal Year
2000
Total Cost
$340,085
Indirect Cost

  Institution

Name
Salk Institute for Biological Studies
Department
Type
DUNS #
005436803
City
La Jolla
State
CA
Country
United States
Zip Code
92037

  Publications

Zarrinpar, Amir; Chaix, Amandine; Xu, Zhenjiang Z et al. (2018) Antibiotic-induced microbiome depletion alters metabolic homeostasis by affecting gut signaling and colonic metabolism. Nat Commun 9:2872
Ramaswamy, Suvasini; Tonnu, Nina; Menon, Tushar et al. (2018) Autologous and Heterologous Cell Therapy for Hemophilia B toward Functional Restoration of Factor IX. Cell Rep 23:1565-1580
Hsu, Cynthia L; Lee, Elian X; Gordon, Kara L et al. (2018) MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB. Nat Commun 9:942
Sonntag, Tim; Vaughan, Joan M; Montminy, Marc (2018) 14-3-3 proteins mediate inhibitory effects of cAMP on salt-inducible kinases (SIKs). FEBS J 285:467-480
Herzig, Sébastien; Shaw, Reuben J (2018) AMPK: guardian of metabolism and mitochondrial homeostasis. Nat Rev Mol Cell Biol 19:121-135
Sweeney, Lora B; Bikoff, Jay B; Gabitto, Mariano I et al. (2018) Origin and Segmental Diversity of Spinal Inhibitory Interneurons. Neuron 97:341-355.e3
Hartmann, Phillipp; Hochrath, Katrin; Horvath, Angela et al. (2018) Modulation of the intestinal bile acid/farnesoid X receptor/fibroblast growth factor 15 axis improves alcoholic liver disease in mice. Hepatology 67:2150-2166
Glustrom, Leslie W; Lyon, Kenneth R; Paschini, Margherita et al. (2018) Single-stranded telomere-binding protein employs a dual rheostat for binding affinity and specificity that drives function. Proc Natl Acad Sci U S A 115:10315-10320
Giraddi, Rajshekhar R; Chung, Chi-Yeh; Heinz, Richard E et al. (2018) Single-Cell Transcriptomes Distinguish Stem Cell State Changes and Lineage Specification Programs in Early Mammary Gland Development. Cell Rep 24:1653-1666.e7
Ma, Jiao; Saghatelian, Alan; Shokhirev, Maxim Nikolaievich (2018) The influence of transcript assembly on the proteogenomics discovery of microproteins. PLoS One 13:e0194518

Showing the most recent 10 out of 457 publications