The central focus of the Cytogenetics Shared Resource (CYT) is to provide high quality cytogenetic and molecular cytogenetic analysis of human and animal model research samples in a timely, cost-effective manner for members of the Mayo Clinic Cancer Center (MCCC) and other investigators within the Mayo research community. External requests are accommodated with discretion. The highly specialized cytogenetic and molecular cytogenetic services and equipment utilized to provide such services to MCCC users would be impractical to institute into multiple individual laboratories. These services are ideally provided in a central location and in a Shared Resource environment, which eliminates the need to duplicate expensive equipment and technologist training and analysis time. Services within the CYT include: Cell Culture, Routine Chromosome Analysis, DNA and RNA fluorescence in situ Hybridization (FISH), and Custom DNA Probe Production as well as RNA FISH Probe Production and a Tyramide Signal Amplification (TSA) protocol. Together, with the investigator, the CYT assists in determining how these techniques can be utilized to meet the evolving needs of their research studies. In addition to the test menu described above, the CYT staff also provides specialized training to investigators for FISH set-up, and microscope use in an effort to minimize investigator cost and economize technologist time. The staff that makes up the CYT have over 75 years of combined cytogenetic and molecular cytogenetic expertise. The capability provided by the CYT is not present in any other Shared Resource facility or research laboratory at Mayo Clinic. The 6 highly qualified and experienced staff members (3.6 FTE) provide expert consultation in experimental design, troubleshooting, modification of experiments, and interpretation of results. The staff also serves to help facilitate the translation of molecular cytogenetic discoveries into clinically available diagnostic assays. In the past grant cycle (2013- 2017), the CYT had a total of 208 users, representing 10 of the 10 MCCC Programs active at the time. Among our 208 users, 84 (40.4%) are MCCC members. Of these 84 investigators, 57 (68%) have active peer- reviewed funding. During these past 5 years, the CYT supported the production of 36 peer-reviewed publications.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee I - Transistion to Independence (NCI)
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Mayo Clinic, Rochester
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Lu, Yingchang; Beeghly-Fadiel, Alicia; Wu, Lang et al. (2018) A Transcriptome-Wide Association Study Among 97,898 Women to Identify Candidate Susceptibility Genes for Epithelial Ovarian Cancer Risk. Cancer Res 78:5419-5430
Dasari, Surendra; Newsom, Sean A; Ehrlicher, Sarah E et al. (2018) Remodeling of skeletal muscle mitochondrial proteome with high-fat diet involves greater changes to ?-oxidation than electron transfer proteins in mice. Am J Physiol Endocrinol Metab 315:E425-E434
Nowsheen, Somaira; Aziz, Khaled; Aziz, Asef et al. (2018) L3MBTL2 orchestrates ubiquitin signalling by dictating the sequential recruitment of RNF8 and RNF168 after DNA damage. Nat Cell Biol 20:455-464
Razidlo, Gina L; Burton, Kevin M; McNiven, Mark A (2018) Interleukin-6 promotes pancreatic cancer cell migration by rapidly activating the small GTPase CDC42. J Biol Chem 293:11143-11153
Wu, Dongyan; Yang, Haitao; Winham, Stacey J et al. (2018) Mediation analysis of alcohol consumption, DNA methylation, and epithelial ovarian cancer. J Hum Genet 63:339-348
Leon-Ferre, Roberto A; Polley, Mei-Yin; Liu, Heshan et al. (2018) Impact of histopathology, tumor-infiltrating lymphocytes, and adjuvant chemotherapy on prognosis of triple-negative breast cancer. Breast Cancer Res Treat 167:89-99
Jahanseir, Khadijeh; Xing, Deyin; Greipp, Patricia T et al. (2018) PDGFB Rearrangements in Dermatofibrosarcoma Protuberans of the Vulva: A Study of 11 Cases Including Myxoid and Fibrosarcomatous Variants. Int J Gynecol Pathol 37:537-546
Painter, Jodie N; O'Mara, Tracy A; Morris, Andrew P et al. (2018) Genetic overlap between endometriosis and endometrial cancer: evidence from cross-disease genetic correlation and GWAS meta-analyses. Cancer Med 7:1978-1987
Yu, Jia; Qin, Bo; Moyer, Ann M et al. (2018) DNA methyltransferase expression in triple-negative breast cancer predicts sensitivity to decitabine. J Clin Invest 128:2376-2388
Sugihara, Takaaki; Werneburg, Nathan W; Hernandez, Matthew C et al. (2018) YAP Tyrosine Phosphorylation and Nuclear Localization in Cholangiocarcinoma Cells Are Regulated by LCK and Independent of LATS Activity. Mol Cancer Res 16:1556-1567

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