IMMUNOGEONOMICS AND FLOW CYTOMETRY SHARED RESOURCE The Immunogenomics and Flow Cytometry SR (IMCF) is a hybrid core that provides access to state-of-the-art techniques such as mass cytometry and immunogenomics analysis of bulk and single cell next generation sequencing data coupled with a high utilization Flow Cytometry facility with a 30-year LCCC history. New immune monitoring capabilities were developed in response to investigator needs, particularly for the analysis of immune responses in preclinical tumor models and human clinical research. The immunogenomics component offers a full suite of immunogenomics techniques, including immune gene signature and pathway analysis, cell phenotype deconvolution, MHC haplotype prediction, neoantigen prediction, T cell and B cell repertoire profiling, and systems level analyses such as cytokine network analysis and integrated survival modeling. This is joined by an investment in CyTOF for expanded panel analysis of cellular phenotypes. This component currently supports correlative science for tissues being collected from eight immunotherapy trials and multiple in vivo animal models of cancer. In addition to these new capabilities, the facility also houses a full complement of highly used analytic flow cytometers and cell sorters for both mouse and human work, New purchases in addition the CyTOF are two new spectral analytic machines. Most important for the research community, all SR components have expert staff to support investigators in experimental design, sample processing, data acquisition, and downstream analysis. IMCF is led by Dr. Vincent, MD, an expert in immunogenomics and Dr. Fisher, PhD who has led the LCC Flow facility since 2010 and has over 30 years? experience in flow cytometry. The budget requested ($211,126) is a small proportion of the total operating budget of $1.7M. Flow cytometry is a traditionally heavily used facility; 80 members were users in fiscal year 2019.

National Institute of Health (NIH)
National Cancer Institute (NCI)
Center Core Grants (P30)
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Subcommittee I - Transistion to Independence (NCI)
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University of North Carolina Chapel Hill
Chapel Hill
United States
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Mayer, Deborah K; Landucci, Gina; Awoyinka, Lola et al. (2018) SurvivorCHESS to increase physical activity in colon cancer survivors: can we get them moving? J Cancer Surviv 12:82-94
Huo, Dezheng; Perou, Charles M; Olopade, Olufunmilayo I (2018) Reported Biologic Differences in Breast Cancer by Race Due to Disparities in Screening-Reply. JAMA Oncol 4:883-884
Howe, Chanelle J; Robinson, Whitney R (2018) Survival-related Selection Bias in Studies of Racial Health Disparities: The Importance of the Target Population and Study Design. Epidemiology 29:521-524
Byrne, James D; Yeh, Jen Jen; DeSimone, Joseph M (2018) Use of iontophoresis for the treatment of cancer. J Control Release 284:144-151
Wilkin, Timothy J; Chen, Huichao; Cespedes, Michelle S et al. (2018) A Randomized, Placebo-Controlled Trial of the Quadrivalent Human Papillomavirus Vaccine in Human Immunodeficiency Virus-Infected Adults Aged 27 Years or Older: AIDS Clinical Trials Group Protocol A5298. Clin Infect Dis 67:1339-1346
Siegel, Marni B; He, Xiaping; Hoadley, Katherine A et al. (2018) Integrated RNA and DNA sequencing reveals early drivers of metastatic breast cancer. J Clin Invest 128:1371-1383
Ubil, Eric; Caskey, Laura; Holtzhausen, Alisha et al. (2018) Tumor-secreted Pros1 inhibits macrophage M1 polarization to reduce antitumor immune response. J Clin Invest 128:2356-2369
Hamad, Ahmad; Iweala, Onyinye I; Henderson, Cory et al. (2018) Recurrent anaphylaxis during cardiac catheterization due to ethylene oxide. J Allergy Clin Immunol Pract 6:2148-2150
Ho, G-T; Aird, R E; Liu, B et al. (2018) MDR1 deficiency impairs mitochondrial homeostasis and promotes intestinal inflammation. Mucosal Immunol 11:120-130
Pearce, Oliver M T; Delaine-Smith, Robin M; Maniati, Eleni et al. (2018) Deconstruction of a Metastatic Tumor Microenvironment Reveals a Common Matrix Response in Human Cancers. Cancer Discov 8:304-319

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