Transgenic and knockout mice have played a very important role in biomedical research for the development of animal models for human diseases and for addressing many different biological questions. The transgenic mouse core facility of the NYU School of Medicine combines the services and resources of the Skirball Institute and the Cancer Institute. The TgESCF currently provides four basic services: production of transgenic mice by zygote injection, production of ES cell chimeras by blastocyst injection or morula aggregation, rederivation of mouse strains, and sperm cryopreservation. Over the next 5 years, the transgenic facility will continue to provide these services, but will also provide new services such as the generation and genotyping of knockout ES cell lines, embryo cyropreservation, the purification of DMA constructs, and assisted reproduction techniques. In the past year, transgenic mice have been produced using inbred FVB/N and C57BL/6 mice, and ES cell chimeras have been produced by injection of ES cells into C57BL/6 blastocysts. To enable investigators to house pathogen-free animals in the Skirball animal facility, rederivation of mouse strains has continued to be important, especially as new staff are recruited who work with mice. Over the past 10 years the .transgenic facility has generated -3,600 transgenic founders from ~670 DMA constructs (including BAC-based constructs), generated ES cell chimeras from -370 ES cell knockout lines and rederived -460 mutant lines. During the period of the current Cancer Center Support Grant (2002-2006), 251 DNA constructs were injected and 877 founders were obtained, chimeras were made from 143 targeted ES cell clones, and 261 mouse strains were rederived. We also froze sperm from 17 mutant mouse strains. In addition to these services, the core director and coordinator provided researchers with support related to work with transgenic and knockout mice, such as training in mouse husbandry and consultation in transgenic and ES cell approaches.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA016087-31
Application #
8232201
Study Section
Subcommittee G - Education (NCI)
Project Start
Project End
Budget Start
2011-03-01
Budget End
2012-02-29
Support Year
31
Fiscal Year
2011
Total Cost
$117,908
Indirect Cost
Name
New York University
Department
Type
DUNS #
121911077
City
New York
State
NY
Country
United States
Zip Code
10016
Winer, Benjamin Y; Shirvani-Dastgerdi, Elham; Bram, Yaron et al. (2018) Preclinical assessment of antiviral combination therapy in a genetically humanized mouse model for hepatitis delta virus infection. Sci Transl Med 10:
Ruggles, Kelly V; Wang, Jincheng; Volkova, Angelina et al. (2018) Changes in the Gut Microbiota of Urban Subjects during an Immersion in the Traditional Diet and Lifestyle of a Rainforest Village. mSphere 3:
MariƩ, Isabelle J; Chang, Hao-Ming; Levy, David E (2018) HDAC stimulates gene expression through BRD4 availability in response to IFN and in interferonopathies. J Exp Med 215:3194-3212
Gupta, Ankit; Xu, Jing; Lee, Shirley et al. (2018) Facile target validation in an animal model with intracellularly expressed monobodies. Nat Chem Biol 14:895-900
Lee, Hyun-Wook; Park, Sung-Hyun; Weng, Mao-Wen et al. (2018) E-cigarette smoke damages DNA and reduces repair activity in mouse lung, heart, and bladder as well as in human lung and bladder cells. Proc Natl Acad Sci U S A 115:E1560-E1569
Evensen, Nikki A; Madhusoodhan, P Pallavi; Meyer, Julia et al. (2018) MSH6 haploinsufficiency at relapse contributes to the development of thiopurine resistance in pediatric B-lymphoblastic leukemia. Haematologica 103:830-839
Formenti, Silvia C; Rudqvist, Nils-Petter; Golden, Encouse et al. (2018) Radiotherapy induces responses of lung cancer to CTLA-4 blockade. Nat Med 24:1845-1851
Sun, Qi; Rabbani, Piul; Takeo, Makoto et al. (2018) Dissecting Wnt Signaling for Melanocyte Regulation during Wound Healing. J Invest Dermatol 138:1591-1600
Gagner, Jean-Pierre; Zagzag, David (2018) Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection. Methods Mol Biol 1741:209-220
Xu, Yang; Taylor, Paul; Andrade, Joshua et al. (2018) Pathologic Oxidation of PTPN12 Underlies ABL1 Phosphorylation in Hereditary Leiomyomatosis and Renal Cell Carcinoma. Cancer Res 78:6539-6548

Showing the most recent 10 out of 1170 publications