Specific interactions between biological macromolecules such as proteins, oligonucleotides, and oligosaccharides provide the chemical foundation for all cellular processes. Characterizing these interactions is essential to defining the biological function of a macromolecule at the molecular level. The role of the Protein Interaction Shared Resource is to provide Cancer Center researchers with easy access to the advanced technologies used in characterizing binding interactions. Currently, a BIACORE 2000 optical biosensor is used to define the assembly state, affinity, a kinetics of an interaction. In a typical biosensor experiment one of the interacting molecules is immobilized onto a surface and the second is passed over this surface in solution. When the molecules interact to form a complex, a response is registered using the optical phenomena of surface plasmon resonance. Since binding reactions are monitored in real time, it is possible to interpret kinetic information about the interaction. Given the complexities involved in biosensor analysis, investigators work closely with the facility's director, Dr. David Myszka, to ensure that experiments are designed properly and that the data are interpreted correctly.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA042014-13
Application #
6334951
Study Section
Project Start
2000-07-25
Project End
2001-04-30
Budget Start
Budget End
Support Year
13
Fiscal Year
2000
Total Cost
Indirect Cost
Name
University of Utah
Department
Type
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Wu, Yelena P; Nagelhout, Elizabeth; Aspinwall, Lisa G et al. (2018) A novel educational intervention targeting melanoma risk and prevention knowledge among children with a familial risk for melanoma. Patient Educ Couns 101:452-459
Li, Lian; Yang, Jiyuan; Wang, Jiawei et al. (2018) Amplification of CD20 Cross-Linking in Rituximab-Resistant B-Lymphoma Cells Enhances Apoptosis Induction by Drug-Free Macromolecular Therapeutics. ACS Nano 12:3658-3670
Ferris, Elliott; Abegglen, Lisa M; Schiffman, Joshua D et al. (2018) Accelerated Evolution in Distinctive Species Reveals Candidate Elements for Clinically Relevant Traits, Including Mutation and Cancer Resistance. Cell Rep 22:2742-2755
Tiburcio, Patricia D B; Xiao, Bing; Chai, Yi et al. (2018) IDH1R132H is intrinsically tumor-suppressive but functionally attenuated by the glutamate-rich cerebral environment. Oncotarget 9:35100-35113
Ou, Judy Y; Fowler, Brynn; Ding, Qian et al. (2018) A statewide investigation of geographic lung cancer incidence patterns and radon exposure in a low-smoking population. BMC Cancer 18:115
Liang, Wenjie; Yang, Pengfei; Huang, Rui et al. (2018) A Combined Nomogram Model to Preoperatively Predict Histologic Grade in Pancreatic Neuroendocrine Tumors. Clin Cancer Res :
Vázquez-Arreguín, Karina; Maddox, Jessica; Kang, Jinsuk et al. (2018) BRCA1 through Its E3 Ligase Activity Regulates the Transcription Factor Oct1 and Carbohydrate Metabolism. Mol Cancer Res 16:439-452
Peres, Lauren C; Cushing-Haugen, Kara L; Anglesio, Michael et al. (2018) Histotype classification of ovarian carcinoma: A comparison of approaches. Gynecol Oncol 151:53-60
Zeng, Tao; Fleming, Aaron M; Ding, Yun et al. (2018) Nanopore Analysis of the 5-Guanidinohydantoin to Iminoallantoin Isomerization in Duplex DNA. J Org Chem 83:3973-3978
Himbert, Caroline; Ose, Jennifer; Nattenmüller, Johanna et al. (2018) Body fatness, adipose tissue compartments and biomarkers of inflammation and angiogenesis in colorectal cancer: the ColoCare Study. Cancer Epidemiol Biomarkers Prev :

Showing the most recent 10 out of 1193 publications