Specific interactions between biological macromolecules such as proteins, oligonucleotides, and oligosaccharides provide the chemical foundation for all cellular processes. Characterizing these interactions is essential to defining the biological function of a macromolecule at the molecular level. The role of the Protein Interaction Shared Resource is to provide Cancer Center researchers with easy access to the advanced technologies used in characterizing binding interactions. Currently, a BIACORE 2000 optical biosensor is used to define the assembly state, affinity, a kinetics of an interaction. In a typical biosensor experiment one of the interacting molecules is immobilized onto a surface and the second is passed over this surface in solution. When the molecules interact to form a complex, a response is registered using the optical phenomena of surface plasmon resonance. Since binding reactions are monitored in real time, it is possible to interpret kinetic information about the interaction. Given the complexities involved in biosensor analysis, investigators work closely with the facility's director, Dr. David Myszka, to ensure that experiments are designed properly and that the data are interpreted correctly.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Center Core Grants (P30)
Project #
5P30CA042014-13
Application #
6334951
Study Section
Project Start
2000-07-25
Project End
2001-04-30
Budget Start
Budget End
Support Year
13
Fiscal Year
2000
Total Cost
Indirect Cost
Name
University of Utah
Department
Type
DUNS #
City
Salt Lake City
State
UT
Country
United States
Zip Code
84112
Fuller, Andrew K; Bice, Benjamin D; Venancio, Ashlee R et al. (2018) A Method to Define the Effects of Environmental Enrichment on Colon Microbiome Biodiversity in a Mouse Colon Tumor Model. J Vis Exp :
Wang, Sophia S; Carrington, Mary; Berndt, Sonja I et al. (2018) HLA Class I and II Diversity Contributes to the Etiologic Heterogeneity of Non-Hodgkin Lymphoma Subtypes. Cancer Res 78:4086-4096
Faham, Najme; Zhao, Ling; Welm, Alana L (2018) mTORC1 is a key mediator of RON-dependent breast cancer metastasis with therapeutic potential. NPJ Breast Cancer 4:36
Rengifo-Cam, William; Shepherd, Hailey M; Jasperson, Kory W et al. (2018) Colon Pathology Characteristics in Li-Fraumeni Syndrome. Clin Gastroenterol Hepatol 16:140-141
Solomon, Benjamin L; Garrido-Laguna, Ignacio (2018) Upper gastrointestinal malignancies in 2017: current perspectives and future approaches. Future Oncol 14:947-962
Gaffney, David K; Hashibe, Mia; Kepka, Deanna et al. (2018) Too many women are dying from cervix cancer: Problems and solutions. Gynecol Oncol 151:547-554
Hardy, Christopher M; Burke, Molly K; Everett, Logan J et al. (2018) Genome-Wide Analysis of Starvation-Selected Drosophila melanogaster-A Genetic Model of Obesity. Mol Biol Evol 35:50-65
Gilcrease, Eddie B; Casjens, Sherwood R (2018) The genome sequence of Escherichia coli tailed phage D6 and the diversity of Enterobacteriales circular plasmid prophages. Virology 515:203-214
Park, Jihye; Blackburn, Brenna E; Rowe, Kerry et al. (2018) Rural-metropolitan disparities in ovarian cancer survival: a statewide population-based study. Ann Epidemiol 28:377-384
Fowler, Brynn; Ding, Qian; Pappas, Lisa et al. (2018) Utah Cancer Survivors: A Comprehensive Comparison of Health-Related Outcomes Between Survivors and Individuals Without a History of Cancer. J Cancer Educ 33:214-221

Showing the most recent 10 out of 1193 publications