We have recently demonstrated that the bitter taste receptor T2R38 is expressed in the upper airway and is activated by acyl-homoserine lactones (AHLs): quorum-sensing molecules secreted by Pseudomonas aeruginosa and other gram-negative bacteria. Furthermore, we found that T2R38 regulates human upper airway innate defenses through nitric oxide production, which stimulates mucociliary clearance and has direct antibacterial effects. Moreover, common polymorphisms of the TAS2R38 gene, correlating with taste sensitivity to the molecule PTC, are linked to significant differences in the ability of uppe respiratory cells to clear and kill bacteria in response to AHLs. Based on these results, our central hypothesis is that the bitter taste receptor T2R38 expressed in the upper respiratory epithelium constitutes a sentinel defense network for the detection and clearance of microbes and that genetic variation in T2R38 contributes to individual differences in airway defensive capabilities. To test this hypothesis, we will: (1) determine whether TAS2R38 genotype, PTC taste sensitivity, and allele-specific expression correlate with symptom severity, disease burden, and interventional outcomes of chronic rhinosinusitis (CRS), and whether TAS2R38 polymorphisims not activated by AHLs are overrepresented in a cohort of CRS patient, and (2) identify other micorbial products that activate the common genetic variants of TAS2R38. The goal of these aims is to determine the role the bitter taste receptor T2R38 plays in upper airway defense and how common polymorphisms of this receptor contribute to upper airway disease. Furthermore, we will determine whether bitter taste sensitivity can be used as an indicator for disease severity in patients with chronic rhinosinusitis, and whether pharmacologic manipulation of this pathway has therapeutic potential.
All people inhale harmful bacteria through the nose. In some, this results in respiratory infections. We propose that the bitter taste receptor T2R38 expressed in the airway senses bitter chemicals secreted by bacteria. When stimulated, this receptor triggers an antimicrobial response and eradicates the offending microbes. Thus, genetic differences in bitter taste receptor functionality may contribute to differences in the predisposition to airway infections.
|Patel, Neil N; Kohanski, Michael A; Maina, Ivy W et al. (2018) Solitary chemosensory cells producing interleukin-25 and group-2 innate lymphoid cells are enriched in chronic rhinosinusitis with nasal polyps. Int Forum Allergy Rhinol :|
|Earl, Joshua P; Adappa, Nithin D; Krol, Jaroslaw et al. (2018) Species-level bacterial community profiling of the healthy sinonasal microbiome using Pacific Biosciences sequencing of full-length 16S rRNA genes. Microbiome 6:190|
|Brooks, Steven G; Trope, Michal; Blasetti, Mariel et al. (2018) Preoperative Lund-Mackay computed tomography score is associated with preoperative symptom severity and predicts quality-of-life outcome trajectories after sinus surgery. Int Forum Allergy Rhinol 8:668-675|
|Patel, Neil N; Workman, Alan D; Cohen, Noam A (2018) Role of Taste Receptors as Sentinels of Innate Immunity in the Upper Airway. J Pathog 2018:9541987|
|Workman, Alan D; Kohanski, Michael A; Cohen, Noam A (2018) Biomarkers in Chronic Rhinosinusitis with Nasal Polyps. Immunol Allergy Clin North Am 38:679-692|
|Triantafillou, Vasiliki; Workman, Alan D; Kohanski, Michael A et al. (2018) Taste Receptor Polymorphisms and Immune Response: A Review of Receptor Genotypic-Phenotypic Variations and Their Relevance to Chronic Rhinosinusitis. Front Cell Infect Microbiol 8:64|
|Douglas, Jennifer E; Mansfield, Corrine J; Arayata, Charles J et al. (2018) Taste Exam: A Brief and Validated Test. J Vis Exp :|
|Workman, Alan D; Maina, Ivy W; Brooks, Steven G et al. (2018) The Role of Quinine-Responsive Taste Receptor Family 2 in Airway Immune Defense and Chronic Rhinosinusitis. Front Immunol 9:624|
|Workman, Alan D; Brooks, Steven G; Kohanski, Michael A et al. (2018) Bitter and sweet taste tests are reflective of disease status in chronic rhinosinusitis. J Allergy Clin Immunol Pract 6:1078-1080|
|Workman, Alan D; Carey, Ryan M; Kohanski, Michael A et al. (2017) Relative susceptibility of airway organisms to antimicrobial effects of nitric oxide. Int Forum Allergy Rhinol 7:770-776|
Showing the most recent 10 out of 34 publications