The recent advances in proteomic instrumentation, methods and informatics have created exciting opportunities in all fields of diabetes research. The information on protein identification and structure provided by mass spectrometry is applicable to nearly all aspects of diabetes and its complications. Mass spectrometry-based proteomics has accelerated the identification of posttranslational modifications, including phosphorylation-site mapping, identification of protein-protein interactions, and changes in protein abundance or compartmentalization, just to name a few examples. However, the rapid rates of growth and change in proteomic technologies have also led to challenges in the translation and availability of these technologies to researchers, from new postdoctoral research fellows to established investigators who are not directly involved in this field. Although many of the fundamental principles of mass spectrometry are relatively straightforward, successful mass spectrometry-based proteomic analysis requires the combination of appropriate experimental design, access to state-of-the-art instrumentation, rigorous analysis of spectral data, and for some studies, bioinformatics tools to manage and interpret large datasets. Indeed, mass spectrometry-based proteomics is a multi-step process, and study design, from the perspectives of mass spectrometry data acquisition and interpretation, plays an important role in experimental success. The overall objective of the Proteomics Core is to provide Joslin researchers with assistance through the workflow of proteomics studies, including experimental design, sample preparation, mass spectrometric analysis, data analysis and interpretation, and bioinformatic tools. The specific objectives of Joslin's Proteomics Core are the following: 1) To assist and provide training in experimental design for proteomics studies. 2) To provide routine and custom mass spectroscopy-based proteomic analyses. 3) To assist with mass spectra analysis, interpretation, and database matching. 4) To develop a results database and incorporate access to bioinformatic tools for the analysis of proteomics data into the results database.

National Institute of Health (NIH)
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Center Core Grants (P30)
Project #
Application #
Study Section
Special Emphasis Panel (ZDK1)
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Joslin Diabetes Center
United States
Zip Code
Panduro, Marisella; Benoist, Christophe; Mathis, Diane (2018) Treg cells limit IFN-? production to control macrophage accrual and phenotype during skeletal muscle regeneration. Proc Natl Acad Sci U S A 115:E2585-E2593
McGill, Dayna E; Volkening, Lisa K; Pober, David M et al. (2018) Depressive Symptoms at Critical Times in Youth With Type 1 Diabetes: Following Type 1 Diabetes Diagnosis and Insulin Pump Initiation. J Adolesc Health 62:219-225
Katz, Michelle L; Guo, Zijing; Laffel, Lori M (2018) Management of Hypertension and High Low-Density Lipoprotein in Pediatric Type 1 Diabetes. J Pediatr 197:140-146.e12
Stanford, Kristin I; Goodyear, Laurie J (2018) Muscle-Adipose Tissue Cross Talk. Cold Spring Harb Perspect Med 8:
Altindis, Emrah; Cai, Weikang; Sakaguchi, Masaji et al. (2018) Viral insulin-like peptides activate human insulin and IGF-1 receptor signaling: A paradigm shift for host-microbe interactions. Proc Natl Acad Sci U S A 115:2461-2466
Yoon, Sujung; Kim, Jungyoon; Musen, Gail et al. (2018) Prefronto-temporal white matter microstructural alterations 20?years after the diagnosis of type 1 diabetes mellitus. Pediatr Diabetes 19:478-485
Silva, Paolo S; Gupta, Aditi; Ajlan, Radwan S et al. (2018) Ultrawide field scanning laser ophthalmoscopy imaging of lipemia retinalis. Acta Ophthalmol 96:e643-e646
Ewald, Collin Yvès; Castillo-Quan, Jorge Iván; Blackwell, T Keith (2018) Untangling Longevity, Dauer, and Healthspan in Caenorhabditis elegans Insulin/IGF-1-Signalling. Gerontology 64:96-104
Viana-Huete, Vanesa; Guillén, Carlos; García, Gema et al. (2018) Male Brown Fat-Specific Double Knockout of IGFIR/IR: Atrophy, Mitochondrial Fission Failure, Impaired Thermogenesis, and Obesity. Endocrinology 159:323-340
Peterson, Claire M; Young-Hyman, Deborah; Fischer, Sarah et al. (2018) Examination of Psychosocial and Physiological Risk for Bulimic Symptoms in Youth With Type 1 Diabetes Transitioning to an Insulin Pump: A Pilot Study. J Pediatr Psychol 43:83-93

Showing the most recent 10 out of 1120 publications