Core - Zebrafish

Zon, Leonard Ira

Abstract

The zebrafish is a relatively new and extremely powerful genetic system for studying vertebrate development (Driever et al. 1996). As zebrafish are small, >70 can be maintained in a five gallon tank. Each mother produces >400 eggs weekly. Hence, large-scale genetics can be performed with ease. At Children's Hospital we have pioneered use of the zebrafish system for hematopoiesis. Two large scale screens for developmental mutants have produced >50 independent mutants affecting blood cell formation (Ransom et. al. 1996). These mutants have been collected at Children's Hospital. Presently, this CORE is a repository for zebrafish mutants of hematopoiesis, and supports their characterization and distribution.

Funding Agency

• Agency: National Institute of Health (NIH)
• Institute: National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
• Type: Center Core Grants (P30)
• Project #: 5P30DK049216-08
• Application #: 6496293
• Project Start: 2001-09-01
• Project End: 2002-08-31
• Support Year: 8
• Fiscal Year: 2001

Institution

• Name: Children's Hospital Boston
• DUNS #: 076593722
• City: Boston
• State: MA
• Country: United States
• Zip Code: 02115

Publications

Vo, Linda T; Kinney, Melissa A; Liu, Xin et al. (2018) Regulation of embryonic haematopoietic multipotency by EZH1. Nature 553:506-510

Mandelbaum, Joseph; Shestopalov, Ilya A; Henderson, Rachel E et al. (2018) Zebrafish blastomere screen identifies retinoic acid suppression of MYB in adenoid cystic carcinoma. J Exp Med 215:2673-2685

Yamauchi, Takuji; Masuda, Takeshi; Canver, Matthew C et al. (2018) Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS. Cancer Cell 33:386-400.e5

Blaser, Bradley W; Zon, Leonard I (2018) Making HSCs in vitro: don't forget the hemogenic endothelium. Blood 132:1372-1378

Cesana, Marcella; Guo, Michael H; Cacchiarelli, Davide et al. (2018) A CLK3-HMGA2 Alternative Splicing Axis Impacts Human Hematopoietic Stem Cell Molecular Identity throughout Development. Cell Stem Cell 22:575-588.e7

Blaser, Bradley W; Moore, Jessica L; Hagedorn, Elliott J et al. (2017) CXCR1 remodels the vascular niche to promote hematopoietic stem and progenitor cell engraftment. J Exp Med 214:1011-1027

Perlin, Julie R; Robertson, Anne L; Zon, Leonard I (2017) Efforts to enhance blood stem cell engraftment: Recent insights from zebrafish hematopoiesis. J Exp Med 214:2817-2827

Doulatov, Sergei; Vo, Linda T; Macari, Elizabeth R et al. (2017) Drug discovery for Diamond-Blackfan anemia using reprogrammed hematopoietic progenitors. Sci Transl Med 9:

Lessard, Samuel; Francioli, Laurent; Alfoldi, Jessica et al. (2017) Human genetic variation alters CRISPR-Cas9 on- and off-targeting specificity at therapeutically implicated loci. Proc Natl Acad Sci U S A 114:E11257-E11266

Perlin, Julie R; Sporrij, Audrey; Zon, Leonard I (2017) Blood on the tracks: hematopoietic stem cell-endothelial cell interactions in homing and engraftment. J Mol Med (Berl) 95:809-819