Abstract

The objective of the Electrophysiology and Xenopus Oocyte is to make readily available to Toxicology Center investigators a variety of electrophysiological assays for ion transport and the Xenopus oocyte expression system for transporters and receptors. The rationale for establishing the core is that these techniques require specialized equipment and/or expertise that might not be available otherwise. The core will offer a broad range of assays ranging from flat-sheet, Ussing Chamber Studies to single channel recording and analysis. In addition the core will be equipped to harvest, defolliculate and inject, with RNA, Xenopus oocytes that can be used as an expression system for transporters or receptors. A primary aim of the Center for Membrane Toxicity Studies is to investigate the mechanism for the toxic effects of metals on membrane proteins, transporters and receptors. The Electrophysiology and Xenopus Oocyte Core will provide the equipment and expertise for Center investigators who wish to employ these important technologies in their research.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Environmental Health Sciences (NIEHS)
Type
Center Core Grants (P30)
Project #
5P30ES003828-12
Application #
6239424
Study Section
Project Start
1997-04-01
Project End
1998-03-31
Budget Start
1996-10-01
Budget End
1997-09-30
Support Year
12
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
Mount Desert Island Biological Lab
Department
Type
DUNS #
City
Salisbury Cove
State
ME
Country
United States
Zip Code
04672

  Publications

Hahn, Mark E; Karchner, Sibel I; Merson, Rebeka R (2017) Diversity as Opportunity: Insights from 600 Million Years of AHR Evolution. Curr Opin Toxicol 2:58-71
Telles, Connor J; Decker, Sarah E; Motley, William W et al. (2016) Functional and molecular identification of a TASK-1 potassium channel regulating chloride secretion through CFTR channels in the shark rectal gland: implications for cystic fibrosis. Am J Physiol Cell Physiol 311:C884-C894
Forrest Jr, John N (2016) THE SHARK RECTAL GLAND MODEL: A CHAMPION OF RECEPTOR MEDIATED CHLORIDE SECRETION THROUGH CFTR. Trans Am Clin Climatol Assoc 127:162-175
Stahl, Klaus; Stahl, Maximilian; de Jonge, Hugo R et al. (2015) ANP and CNP activate CFTR expressed in Xenopus laevis oocytes by direct activation of PKA. J Recept Signal Transduct Res 35:493-504
Schwarz, Julia S; de Jonge, Hugo R; Forrest Jr, John N (2015) Value of Organoids from Comparative Epithelia Models. Yale J Biol Med 88:367-74
Kelley, Catherine A; Decker, Sarah E; Silva, Patricio et al. (2014) Gastric inhibitory peptide, serotonin, and glucagon are unexpected chloride secretagogues in the rectal gland of the skate (Leucoraja erinacea). Am J Physiol Regul Integr Comp Physiol 306:R674-80
De Jonge, Hugo R; Tilly, Ben C; Hogema, Boris M et al. (2014) cGMP inhibition of type 3 phosphodiesterase is the major mechanism by which C-type natriuretic peptide activates CFTR in the shark rectal gland. Am J Physiol Cell Physiol 306:C343-53
Christian, Whitney V; Li, Na; Hinkle, Patricia M et al. (2012) ?-Subunit of the Ost?-Ost? organic solute transporter is required not only for heterodimerization and trafficking but also for function. J Biol Chem 287:21233-43
Barnes, D W (2012) Cell and molecular biology of the spiny dogfish Squalus acanthias and little skate Leucoraja erinacea: insights from in vitro cultured cells. J Fish Biol 80:2089-111
Miller, Hilary D; Clark, Bryan W; Hinton, David E et al. (2012) Anchoring ethinylestradiol induced gene expression changes with testicular morphology and reproductive function in the medaka. PLoS One 7:e52479

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