The Molecular Biology Module provides services involved with the production of transgenic, knock-out and knock-in mice and construction of gene constructs used for an array of applications (e.g., in situ hybridization probes), preparation of DNA clones and probes, and consultation or direct service for the design of polymerase chain reaction primers and optimization of reaction conditions. The module also provides limited- scale DNA sequencing, consultation or direct services for phosphorimaging and flow cytometry for cell analysis, and maintenance and oversight of shared molecular biology instrumentation. The module occupies a new laboratory designed for its exclusive use. The module is staffed by two full-time molecular biology technicians with advanced skills in molecular cloning, DNA manipulations, animal husbandry and microinjection. The services of this module enhance the environment for research by making molecular biology an accessible tool for any member of the vision core group. The environment is further enhanced by providing training and support in the use of modern shared instrumentation for molecular biology, including the STORM phosphorimager and Beckman Coulter FC500 flow cytometer.

Agency
National Institute of Health (NIH)
Institute
National Eye Institute (NEI)
Type
Center Core Grants (P30)
Project #
2P30EY002687-27
Application #
6949368
Study Section
Special Emphasis Panel (ZEY1-VSN (03))
Project Start
2005-04-01
Project End
2010-03-31
Budget Start
2005-04-01
Budget End
2006-03-31
Support Year
27
Fiscal Year
2005
Total Cost
$186,622
Indirect Cost
Name
Washington University
Department
Type
DUNS #
068552207
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
Majtan, Tomas; Jones Jr, Wendell; Krijt, Jakub et al. (2018) Enzyme Replacement Therapy Ameliorates Multiple Symptoms of Murine Homocystinuria. Mol Ther 26:834-844
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