The ability to test hypotheses in a variety of neuroscience fields has expanded exponentially due to new and emerging technologies that detect and probe molecular, cellular and physiological events in terminally collected samples or living model organisms with ever increasing power and capabilities. Supported by an NINDS P30 grant since 2003, the University of California San Diego Neuroscience Microscopy Imaging Core has grown into a world-class core and a centerpiece for local neuroscience research. Importantly, the Core serves an otherwise unmet neuroscience research need in microscopy imaging of many laboratories. Its existence has resulted in remarkable yields in productivity, expanded scientific scope and ability to test hypotheses using cutting edge technologies. In this application, we seek to acquire a Zeiss Lightsheet Z.1 microscope for fast, gentle, multiview imaging deep into thick samples such as cleared brain and spinal cord tissue blocks or living organisms. The requested system was chosen because of the high quality of data that it generates, unsurpassed abilities to document events in the nervous system not previously feasible and its ease of use, which is critical for a multi-user core. In addition, we ask for funds to help maintain, operate and support a number of existing cutting edge imaging tools at the Core. On top of 35 NINDS-funded Major User labs with 44 qualifying projects, a wider base of neuroscience investigators benefit from access to our Core. UCSD is a leading institution in neuroscience research, with our neuroscience graduate program consistently ranked among the top in the nation. The strong support of local neuroscience researchers along with that of institutional leadership, the synergies and the economies of scale that the Core has created and the continued P30 grant support will ensure the future success of our Core. In return, our Core supports the mission of the NINDS by serving a wide range of outstanding research programs that aim to gain fundamental knowledge of the nervous system and to reduce the burden of neurological diseases.

Public Health Relevance

Modern neuroscience research requires access to newly emerging technologies and instruments that often would be difficult or impractical to support in individual laboratories. Building its highly successful operation supporting a wide base of outstanding neuroscientists in the last 13 years, the University of California San Diego Neuroscience Microscopy Imaging Core seeks continued P30 funding to maintain and expand its ability to serve an otherwise unmet neuroscience research need. By supporting research programs that seek fundamental knowledge about the nervous system and strive to use that knowledge to identify therapies for neurological diseases, the Core fully aligns with the NINDS mission.

National Institute of Health (NIH)
National Institute of Neurological Disorders and Stroke (NINDS)
Center Core Grants (P30)
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Study Section
Special Emphasis Panel (ZNS1)
Program Officer
Lavaute, Timothy M
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University of California, San Diego
Schools of Medicine
La Jolla
United States
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Kyriakakis, Phillip; Catanho, Marianne; Hoffner, Nicole et al. (2018) Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP+ Reductase Systems Enables Genetically Encoded PhyB Optogenetics. ACS Synth Biol 7:706-717
Kalyanaraman, Hema; Schwaerzer, Gerburg; Ramdani, Ghania et al. (2018) Protein Kinase G Activation Reverses Oxidative Stress and Restores Osteoblast Function and Bone Formation in Male Mice With Type 1 Diabetes. Diabetes 67:607-623
Nelson, Katelyn N; Meyer, April N; Wang, Clark G et al. (2018) Oncogenic driver FGFR3-TACC3 is dependent on membrane trafficking and ERK signaling. Oncotarget 9:34306-34319
Maricic, Igor; Marrero, Idania; Eguchi, Akiko et al. (2018) Differential Activation of Hepatic Invariant NKT Cell Subsets Plays a Key Role in Progression of Nonalcoholic Steatohepatitis. J Immunol 201:3017-3035
Lagarrigue, Frederic; Gingras, Alexandre R; Paul, David S et al. (2018) Rap1 binding to the talin 1 F0 domain makes a minimal contribution to murine platelet GPIIb-IIIa activation. Blood Adv 2:2358-2368
De La Zerda, D J; Stokes, J A; Do, J et al. (2018) Ibuprofen does not reverse ventilatory acclimatization to chronic hypoxia. Respir Physiol Neurobiol 256:29-35
Schaffer, Ashleigh E; Breuss, Martin W; Caglayan, Ahmet Okay et al. (2018) Biallelic loss of human CTNNA2, encoding ?N-catenin, leads to ARP2/3 complex overactivity and disordered cortical neuronal migration. Nat Genet 50:1093-1101
Narang, Aarti; Zheng, Binhai (2018) To Scar or Not to Scar. Trends Mol Med 24:522-524
Patel, Akash; Li, Zhongzhi; Canete, Philip et al. (2018) AxonTracer: a novel ImageJ plugin for automated quantification of axon regeneration in spinal cord tissue. BMC Neurosci 19:8
Yan, Jian; Chen, Shi-An A; Local, Andrea et al. (2018) Histone H3 lysine 4 monomethylation modulates long-range chromatin interactions at enhancers. Cell Res 28:204-220

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