We are constantly looking to employ new methods to enhance theseparation and identification of peptides. We are fortunate to have Dr. David Goodlett as a core director as heis actively involved in the development of new mass spectrometry techniques (please see his biosketch andletter). We have also recruited an internationally recognized expert on mass spectrometry and bioinformatics,Dr. Timothy Veenstra, as an external consultant. These two individuals will insure that Core C provides stateof the art advances to our end users. One future direction will involve incorporating stable isotope labelingmethods, e.g. isotope coded affinity tag (ICAT) or iTRAQ methods, as well as so-called label-free methods asrecently described in Dr. Goodlett's laboratory (Foss et al., In Press, Nature Genetics). This will allow us toprovide information on the relative levels of expression of proteins in a complex and discern bona fidemembers of the complexes from non-specific, contaminating proteins58. In addition, depending on ourresources, it may be possible to provide global proteome analysis as we previously described for mousecortical neurons57 and mouse astrocytes (McBee et al, In Press, Proteomics; see Morrison Biosketch).
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| Young, Jennifer M; Nelson, Jonathan W; Cheng, Jian et al. (2015) Peroxisomal biogenesis in ischemic brain. Antioxid Redox Signal 22:109-20 |
| Wang, David B; Kinoshita, Yoshito; Kinoshita, Chizuru et al. (2015) Loss of endophilin-B1 exacerbates Alzheimer's disease pathology. Brain 138:2005-19 |
| Wang, David B; Kinoshita, Chizuru; Kinoshita, Yoshito et al. (2014) p53 and mitochondrial function in neurons. Biochim Biophys Acta 1842:1186-97 |
| Wang, David B; Uo, Takuma; Kinoshita, Chizuru et al. (2014) Bax interacting factor-1 promotes survival and mitochondrial elongation in neurons. J Neurosci 34:2674-83 |
| Wang, David B (2013) Monophosphoryl lipid A is an lipopolysaccharide-derived Toll-like receptor 4 agonist which may improve Alzheimer's disease pathology. Expert Opin Biol Ther 13:1639-41 |
| Wang, David B; Garden, Gwenn A; Kinoshita, Chizuru et al. (2013) Declines in Drp1 and parkin expression underlie DNA damage-induced changes in mitochondrial length and neuronal death. J Neurosci 33:1357-65 |
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