Abstract

NMS Core: 1. A computer-controlled, motorized upright fluorescence microscope (Zeiss Axioimager), with piezo-controlled stage and microscanning CCD camera. This microscope is well-equipped for Array Tomography data acquisition. 2. An inverted, laser scanning confocal microscope with spectral imaging capability (Zeiss LSM510 Meta) and an environmental control chamber for time-lapse imaging of live cells. 3. Two upright two-photon microscopes on a single large optical table, sharing a pair of Ti:sapphire femtosecond pulsed lasers. Both microscopes are equipped for simultaneous two-photon imaging and twophoton photoactivation. Configurable table optics also allow for simultaenous imaging at dual wavelengths. a. One microscope is designed for in vivo imaging (Ultima IV, Prairie Technologies, Middleton, Wl). b. The other microscope is designed for tissue slice imaging (Ultima XY, Prairie Technologi

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Neurological Disorders and Stroke (NINDS)
Type
Center Core Grants (P30)
Project #
5P30NS069375-02
Application #
8377904
Study Section
National Institute of Neurological Disorders and Stroke Initial Review Group (NSD)
Project Start
Project End
Budget Start
2011-12-01
Budget End
2012-11-30
Support Year
2
Fiscal Year
2012
Total Cost
$158,898
Indirect Cost
$58,330

  Institution

Name
Stanford University
Department
Type
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Kornfeld, Opher S; Qvit, Nir; Haileselassie, Bereketeab et al. (2018) Interaction of mitochondrial fission factor with dynamin related protein 1 governs physiological mitochondrial function in vivo. Sci Rep 8:14034
Zhang, Zhenjie; Marro, Samuele G; Zhang, Yingsha et al. (2018) The fragile X mutation impairs homeostatic plasticity in human neurons by blocking synaptic retinoic acid signaling. Sci Transl Med 10:
Joshi, Amit U; Saw, Nay L; Shamloo, Mehrdad et al. (2018) Drp1/Fis1 interaction mediates mitochondrial dysfunction, bioenergetic failure and cognitive decline in Alzheimer's disease. Oncotarget 9:6128-6143
Stamatakis, Alice M; Schachter, Mike J; Gulati, Srishti et al. (2018) Simultaneous Optogenetics and Cellular Resolution Calcium Imaging During Active Behavior Using a Miniaturized Microscope. Front Neurosci 12:496
Kramer, Nicholas J; Haney, Michael S; Morgens, David W et al. (2018) CRISPR-Cas9 screens in human cells and primary neurons identify modifiers of C9ORF72 dipeptide-repeat-protein toxicity. Nat Genet 50:603-612
Li, Shi-Bin; Nevárez, Natalie; Giardino, William J et al. (2018) Optical probing of orexin/hypocretin receptor antagonists. Sleep 41:
Liu, Qing; Van Bortle, Kevin; Zhang, Yue et al. (2018) Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960
Razavi, Mehdi; Thakor, Avnesh S (2018) An oxygen plasma treated poly(dimethylsiloxane) bioscaffold coated with polydopamine for stem cell therapy. J Mater Sci Mater Med 29:54
Giardino, William J; Eban-Rothschild, Ada; Christoffel, Daniel J et al. (2018) Parallel circuits from the bed nuclei of stria terminalis to the lateral hypothalamus drive opposing emotional states. Nat Neurosci 21:1084-1095
Bennett, F Chris; Bennett, Mariko L; Yaqoob, Fazeela et al. (2018) A Combination of Ontogeny and CNS Environment Establishes Microglial Identity. Neuron 98:1170-1183.e8

Showing the most recent 10 out of 102 publications