This resubmission of our animal resource grant renewal proposal requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded on September 21, 2006. It has the following resource component and research component aims: A. Resource Component Related Aims: (1) To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. (2) To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. (3) To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. B. Research Component Aims: To expand the genetic data available for this species to a) increase the research utility of the species and b) assist in disease management of the colony. This will be done through three research aims. (1) To characterize pig-tailed macaque immunogenetics in detail via MHC class I genotyping. (2) To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. (3) To determine whether either MHC class I allele expression or SNPs are associated with a) SIV-related disease outcomes and b) naturally occurring enterocolitis in pig-tailed macaques.

Public Health Relevance

Specific pathogen free pig-tailed macaques are in short supply in the US at this time, yet they are important animal models in a variety of disease entities. This colony is one of the two such colonies in the US, and will provide animals to NIH funded investigators. The research aims of this project are important to expanding the knowledge base of this macaque species which will increase its utility as an animal model of human disease.

National Institute of Health (NIH)
Office of The Director, National Institutes of Health (OD)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
Application #
Study Section
Special Emphasis Panel (ZRR1-CM-1 (01))
Program Officer
Moro, Manuel H
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Johns Hopkins University
Veterinary Sciences
Schools of Medicine
United States
Zip Code
Mangus, Lisa M; Beck, Sarah E; Queen, Suzanne E et al. (2018) Lymphocyte-Dominant Encephalitis and Meningitis in Simian Immunodeficiency Virus-Infected Macaques Receiving Antiretroviral Therapy. Am J Pathol 188:125-134
Beck, Sarah E; Queen, Suzanne E; Metcalf Pate, Kelly A et al. (2018) An SIV/macaque model targeted to study HIV-associated neurocognitive disorders. J Neurovirol 24:204-212
Vermillion, Meghan S; Lyons, Claire E; Najarro, Kevin M et al. (2017) Immune Activation of Platelets in Response to Serial Phlebotomy in Pigtailed Macaques (Macaca nemestrina). Comp Med 67:360-367
Croteau, Joshua D; Engle, Elizabeth L; Queen, Suzanne E et al. (2017) Marked Enteropathy in an Accelerated Macaque Model of AIDS. Am J Pathol 187:589-604
Shirk, Erin N; Kral, Brian G; Gama, Lucio (2017) Toll-like receptor 2bright cells identify circulating monocytes in human and non-human primates. Cytometry A 91:364-371
Klein, Amanda H; Vyshnevska, Alina; Hartke, Timothy V et al. (2017) Sodium Channel Nav1.8 Underlies TTX-Resistant Axonal Action Potential Conduction in Somatosensory C-Fibers of Distal Cutaneous Nerves. J Neurosci 37:5204-5214
Williams, Dionna W; Engle, Elizabeth L; Shirk, Erin N et al. (2016) Splenic Damage during SIV Infection: Role of T-Cell Depletion and Macrophage Polarization and Infection. Am J Pathol 186:2068-2087
Mangus, Lisa M; Dorsey, Jamie L; Weinberg, Rachel L et al. (2016) Tracking Epidermal Nerve Fiber Changes in Asian Macaques: Tools and Techniques for Quantitative Assessment. Toxicol Pathol 44:904-12
Avalos, Claudia R; Price, Sarah L; Forsyth, Ellen R et al. (2016) Quantitation of Productively Infected Monocytes and Macrophages of Simian Immunodeficiency Virus-Infected Macaques. J Virol 90:5643-5656
Buchanan, Erin L; Espinoza, Diego A; McAlexander, Melissa A et al. (2016) SAMHD1 transcript upregulation during SIV infection of the central nervous system does not associate with reduced viral load. Sci Rep 6:22629

Showing the most recent 10 out of 45 publications