The genomic sequence of the fission yeast S. pombe will soon be published. This yeast provides a powerful genetic and molecular system, which is currently the major focus of study for about 40 laboratories in the U.S., and an even larger number in Europe and Japan. Many of the laboratories in the U.S., including those of Drs. Janet Leatherwood and A. Bruce Futcher, work on the cell cycle, for which S. pombe is an excellent model organism. Spotted cDNA microarrays are a cheap, rapid, efficient method for collecting data and investigating many subjects from cell physiology and transcription circuits to chromosome structure, mRNA splicing, translational regulation, DNA replication control, and meiotic recombination. Here, it is proposed to create a facility for the production and use of S. pombe cDNA microarrays. These microarrays would be available to the community of S. pombe workers in the U.S., and, if resources permit, to workers in other countries. Microarrays would be made at a facility at the SUNY, Stony Brook. Outside users of the facility would do experiments designed for microarray analysis, isolate RNA (or DNA), validate the samples, and convert the RNA to labeled cDNA or label the isolated DNA. This labeled DNA would be sent to the facility, where it would be hybridized to the microarrays, scanned, and subjected to standard forms of analysis. Results would be posted to a Web site for further analysis by the user. Resources for more extensive data analysis would be available on the facility?s Web site.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
5P40RR016320-04
Application #
6771209
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
O'Neill, Raymond R
Project Start
2001-07-16
Project End
2007-06-30
Budget Start
2004-07-01
Budget End
2007-06-30
Support Year
4
Fiscal Year
2004
Total Cost
$208,519
Indirect Cost
Name
State University New York Stony Brook
Department
Genetics
Type
Schools of Medicine
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Zhou, Chunshui; Arslan, Fatih; Wee, Susan et al. (2005) PCI proteins eIF3e and eIF3m define distinct translation initiation factor 3 complexes. BMC Biol 3:14