This is a P40 resource proposal to maintain and expand the Xiphophorus Genetic Stock Center (XGSC), and enhance services provided to research investigators. Xiphophorus is a small freshwater, aquarium fish that has been extensively used in carcinogenesis studies, but also used as a model organism in a variety of different fields of study that transcends several NIH institutes. The XGSC has operated within the United States for over 70 years. The proposed initiatives will greatly enhance the usability of the Xiphophorus model system for research scientists using this system, but also for """"""""non-Xiphophorus"""""""" scientists that envision application of the powerful genetics provided by this model system. The traditional strength of this model involves the non-biased assessment of genetic variability among Xiphophorus species (22 known taxa), and capability of producing fertile interspecies hybrids, allows chromosomal/marker inheritance to be followed in individual backcross hybrid progeny. Complex traits, such as susceptibility to the development of neoplastic disease, or behavioral characteristics, can be studied using Xiphophorus, and underlying genetic factors can be identified. Since research using these fishes is currently expanding within the scientific community, it is timely to enhance the services offered by the Xiphophorus Genetic Stock Center. We propose: 1) To preserve and enhance the Xiphophorus Genetic Stock Center and provide the research community with animals from genetically managed lines in large numbers according to the specifications of each investigator. 2) To establish and maintain extensive and flexible computer databases of fish, and derived samples. These databases will be made available for scientific use. Links and information regarding fish maintenance, current research projects and other XGSC information will be provided via the xiphophorus.org web- site. 3) To continue saturation of the Xiphophorus gene map and development of technologies providing investigators with efficient molecular tools necessary to use this powerful genetic system to approach complex scientific questions, and 4) To establish technology allowing cryopreservation of Xiphophorus sperm samples and thereby provide a preservation program where the XGSC maintains the genetic heritage of all Xiphophorus strains and species.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
5P40RR017072-02
Application #
6623463
Study Section
Special Emphasis Panel (ZRR1-CM-9 (03))
Program Officer
Chang, Michael
Project Start
2002-05-01
Project End
2007-04-30
Budget Start
2003-05-01
Budget End
2004-04-30
Support Year
2
Fiscal Year
2003
Total Cost
$340,267
Indirect Cost
Name
Texas State University-San Marcos
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
074602368
City
San Marcos
State
TX
Country
United States
Zip Code
78666
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Zhang, Ziping; Ju, Zhenlin; Wells, Melissa C et al. (2009) Genomic approaches in the identification of hypoxia biomarkers in model fish species. J Exp Mar Bio Ecol 381:S180-S187
Yang, Huiping; Carmichael, Carrie; Varga, Zoltan M et al. (2007) Development of a simplified and standardized protocol with potential for high-throughput for sperm cryopreservation in zebrafish Danio rerio. Theriogenology 68:128-36
Yang, Huiping; Hazlewood, Leona; Heater, Sheila J et al. (2007) Production of F1 interspecies hybrid offspring with cryopreserved sperm from a live-bearing fish, the swordtail Xiphophorus helleri. Biol Reprod 76:401-6
Heater, Sheila J; Rains, J Douglas; Wells, Melissa C et al. (2007) Perturbation of DNA repair gene expression due to interspecies hybridization. Comp Biochem Physiol C Toxicol Pharmacol 145:156-63
Wood, Simon R; Berwick, Marianne; Ley, Ronald D et al. (2006) UV causation of melanoma in Xiphophorus is dominated by melanin photosensitized oxidant production. Proc Natl Acad Sci U S A 103:4111-5
Yang, Huiping; Hazlewood, Leona; Walter, Ronald B et al. (2006) Effect of osmotic immobilization on refrigerated storage and cryopreservation of sperm from a viviparous fish, the green swordtail Xiphophorus helleri. Cryobiology 52:209-18
Oehlers, Leon P; Perez, Amy N; Walter, Ronald B (2005) Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of 4-sulfophenyl isothiocyanate-derivatized peptides on AnchorChip sample supports using the sodium-tolerant matrix 2,4,6-trihydroxyacetophenone and diammonium citrate. Rapid Commun Mass Spectrom 19:752-8
Pinisetty, D; Huang, C; Dong, Q et al. (2005) Subzero water permeability parameters and optimal freezing rates for sperm cells of the southern platyfish, Xiphophorus maculatus. Cryobiology 50:250-63
Walter, R B; Rains, J D; Russell, J E et al. (2004) A microsatellite genetic linkage map for Xiphophorus. Genetics 168:363-72

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