The comprehensive mission governing the strategy of the Developmental Resource for Biophysical Imaging and Opto-Electronics (DRBIO) is the creation and facilitation of instrumentation technologies that advance capabilities for visualization and measurement of dynamic biomolecular and cellular processes. Thus, the core research aims to create innovations that are developed by """"""""Biomedical Engineering"""""""" into """"""""Technologies for the Study of Molecular and Cellular Structure and Function."""""""" It is the challenges of """"""""impossible""""""""- but crucial - biological problems that motivate each new direction of our core research, leading to developments of instrumentation to facilitate our collaborations and services, the dissemination of the resulting innovations, and to training of participants and clients to support the transfer of the new technologies to the biomedical community. This combination of instrument development and collaboration is efficient and synergistic, with frequent reciprocal intellectual exchanges between collaborators and staff creating new concepts and understanding for both groups. The program comprises three classes of projects: (1) Biomedical instrumentation engineering to advance the effectiveness of biomedical application of the technologies of multiphoton microscopy (MPM) and fluorescence correlation spectroscopy (FCS), invented and already featured in DRBIO. (2) New directions of development of photophysical technologies, focusing particularly on robust optical markers for sensitive single molecule observations, and on in vivo imaging of the intrinsic fluorescence of tissue components. (3) Venturesome biotechnology developments based on new technologies, including optical nanostructures, nonlinear photochemistry and correlation spectroscopy to address several crucial but refractory challenges of biomedical research.

National Institute of Health (NIH)
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Biotechnology Resource Grants (P41)
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Special Emphasis Panel (ZRG1-SSS-7 (40))
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Mclaughlin, Alan Charles
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Cornell University
Engineering (All Types)
Schools of Engineering
United States
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Novakofski, K D; Williams, R M; Fortier, L A et al. (2014) Identification of cartilage injury using quantitative multiphoton microscopy. Osteoarthritis Cartilage 22:355-62
Shui, Bo; Ozer, Abdullah; Zipfel, Warren et al. (2012) RNA aptamers that functionally interact with green fluorescent protein and its derivatives. Nucleic Acids Res 40:e39
Choi, Nak Won; Verbridge, Scott S; Williams, Rebecca M et al. (2012) Phosphorescent nanoparticles for quantitative measurements of oxygen profiles in vitro and in vivo. Biomaterials 33:2710-22
Degala, Satish; Zipfel, Warren R; Bonassar, Lawrence J (2011) Chondrocyte calcium signaling in response to fluid flow is regulated by matrix adhesion in 3-D alginate scaffolds. Arch Biochem Biophys 505:112-7
Kadiri, Lolahon R; Kwan, Alex C; Webb, Watt W et al. (2011) Dopamine-induced oscillations of the pyloric pacemaker neuron rely on release of calcium from intracellular stores. J Neurophysiol 106:1288-98
Morales-Penningston, Nelson F; Wu, Jing; Farkas, Elaine R et al. (2010) GUV preparation and imaging: minimizing artifacts. Biochim Biophys Acta 1798:1324-32
Kwan, Alex C; Dietz, Shelby B; Zhong, Guisheng et al. (2010) Spatiotemporal dynamics of rhythmic spinal interneurons measured with two-photon calcium imaging and coherence analysis. J Neurophysiol 104:3323-33
Kwan, Alex C; Dietz, Shelby B; Webb, Watt W et al. (2009) Activity of Hb9 interneurons during fictive locomotion in mouse spinal cord. J Neurosci 29:11601-13
Kwan, Alex C; Duff, Karen; Gouras, Gunnar K et al. (2009) Optical visualization of Alzheimer's pathology via multiphoton-excited intrinsic fluorescence and second harmonic generation. Opt Express 17:3679-89
Cheshire, Alan M; Kerman, Bilal E; Zipfel, Warren R et al. (2008) Kinetic and mechanical analysis of live tube morphogenesis. Dev Dyn 237:2874-88

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