Abstract

A detailed plan is presented for the development and construction of the required apparatus. The mixing apparatus is a commercial KinTec device that has been widely used and accepted within the biochemistry and biophysics communities. Specific modifications to the rapid-mixing device are proposed to minimize sample volume and X-ray exposure. Specific plans are presented for the construction of the beamline shutter, adjustable slit, electronic shutter, precision motorized table, beam alignment system, exposure cell, and rapid-mixing apparatus. The development of these devices has involved the Chance-Brenowitz team, who have pioneered these methods on the lower flux beamline, Brookhaven scientific and engineering staff, and engineers and physicists in private companies that have had a good deal of experience in working with synchrotron staff, and the biochemistry and biophysics communities ? ?

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Biomedical Imaging and Bioengineering (NIBIB)
Type
Biotechnology Resource Grants (P41)
Project #
5P41EB001979-25
Application #
7121917
Study Section
Special Emphasis Panel (ZRG1-BNP (40))
Program Officer
Mclaughlin, Alan Charles
Project Start
1997-09-30
Project End
2008-08-31
Budget Start
2006-09-01
Budget End
2007-08-31
Support Year
25
Fiscal Year
2006
Total Cost
$991,447
Indirect Cost

  Institution

Name
Case Western Reserve University
Department
Genetics
Type
Schools of Medicine
DUNS #
077758407
City
Cleveland
State
OH
Country
United States
Zip Code
44106

  Publications

Manjasetty, Babu A; Chance, Mark R; Burley, Stephen K et al. (2014) Crystal structure of Clostridium acetobutylicum Aspartate kinase (CaAK): An important allosteric enzyme for amino acids production. Biotechnol Rep (Amst) 3:73-85
Wang, Dong; Ray, Kallol; Collins, Michael J et al. (2013) Nonheme Oxoiron(IV) Complexes of Pentadentate N5 Ligands: Spectroscopy, Electrochemistry, and Oxidative Reactivity. Chem Sci 4:282-291
Oztug Durer, Zeynep A; Kamal, J K Amisha; Benchaar, Sabrina et al. (2011) Myosin binding surface on actin probed by hydroxyl radical footprinting and site-directed labels. J Mol Biol 414:204-16
Yu, Peiqiang (2011) Microprobing the molecular spatial distribution and structural architecture of feed-type sorghum seed tissue (Sorghum Bicolor L.) using the synchrotron radiation infrared microspectroscopy technique. J Synchrotron Radiat 18:790-801
Yu, Peiqiang (2010) Plant-based food and feed protein structure changes induced by gene-transformation, heating and bio-ethanol processing: a synchrotron-based molecular structure and nutrition research program. Mol Nutr Food Res 54:1535-45
Liu, Na; Yu, Peiqiang (2010) Characterization of the microchemical structure of seed endosperm within a cellular dimension among six barley varieties with distinct degradation kinetics, using ultraspatially resolved synchrotron-based infrared microspectroscopy. J Agric Food Chem 58:7801-10
Orban, Tivadar; Gupta, Sayan; Palczewski, Krzysztof et al. (2010) Visualizing water molecules in transmembrane proteins using radiolytic labeling methods. Biochemistry 49:827-34
Liu, Na; Yu, Peiqiang (2010) Using DRIFT molecular spectroscopy with uni- and multivariate spectral techniques to detect protein molecular structure differences among different genotypes of barley. J Agric Food Chem 58:6264-9
Mak, Kin Fai; Shan, Jie; Heinz, Tony F (2010) Electronic structure of few-layer graphene: experimental demonstration of strong dependence on stacking sequence. Phys Rev Lett 104:176404
Gupta, Sayan; Bavro, Vassiliy N; D'Mello, Rhijuta et al. (2010) Conformational changes during the gating of a potassium channel revealed by structural mass spectrometry. Structure 18:839-46

Showing the most recent 10 out of 55 publications