To study protein metabolism in uremia, whole body leucine flux was measured by a primed constant infusion of L(1-33C) leucine in 9 chronic renal failure (CRF) patients on three occasions; twice before and once following the initiation of dialysis treatment. Before dialysis, one leucine flux was measured when the patients were acidotic, and the other, when acidosis was corrected with bicarbonate supplement. All subjects consumed a constant diet for 6 days during each study period. Plasma L(1-33C)leucine and L(1-33C) KIC were measured by gas chromatography/mass spectrometry and expired 13CO2.by isotope mass spectrometry. Leucine kinetics were calculated using standard equations and statistical analyses derived using one-way repeated measures Anova. The pH and PCO2 were [data presented as means+SD] 7.29+0.05, 7.39+0.04 and 7.43+0.05, and plasma total CO2 were 19+3, 26+2, and 31+3 nmol/L, respectively in A, NA and D, corresponding to acidotic, non-acidotic and dialysis period [p=0.87x10-5 and 0.24x10-6]. 33CO2 production rates were 0.48+0.15, 0.31+0.15 and 0.31+0.06 umol/kg/hr, and leucine oxidation were 16+5, 94, and 12+3 umol/kg/hr, respectively, during A, NA and D periods (p=.0002 and 0.005]. Leucine release from protein degradation were 101+12, 95+9 and 113+22, and leucine protein incorporation rate were 85+10, 85+8 and 101+20 umol/kg/hr, respectively, in the A, NA and D periods [p=0.004 and 0.001]. The observations indicate that acidosis led to accelerated leucine oxidation. Uremia per se did not increase protein degradation. Six to 10 weeks following the initiation of dialysis, protein anabolism occurred as evident by a rise in leucine flux and an increase in protein synthesis. These findings underscore the importance of alkali therapy and dialysis in maintaining nutritional status in CRF patients.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000954-20
Application #
5221820
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
20
Fiscal Year
1996
Total Cost
Indirect Cost
Yue, Xuyi; Dhavale, Dhruva D; Li, Junfeng et al. (2018) Design, synthesis, and in vitro evaluation of quinolinyl analogues for ?-synuclein aggregation. Bioorg Med Chem Lett 28:1011-1019
Ohlemacher, Shannon I; Giblin, Daryl E; d'Avignon, D André et al. (2017) Enterobacteria secrete an inhibitor of Pseudomonas virulence during clinical bacteriuria. J Clin Invest 127:4018-4030
Lin, Xiaobo; Racette, Susan B; Ma, Lina et al. (2017) Endogenous Cholesterol Excretion Is Negatively Associated With Carotid Intima-Media Thickness in Humans. Arterioscler Thromb Vasc Biol 37:2364-2369
Ovod, Vitaliy; Ramsey, Kara N; Mawuenyega, Kwasi G et al. (2017) Amyloid ? concentrations and stable isotope labeling kinetics of human plasma specific to central nervous system amyloidosis. Alzheimers Dement 13:841-849
Cade, W Todd; Levy, Philip T; Tinius, Rachel A et al. (2017) Markers of maternal and infant metabolism are associated with ventricular dysfunction in infants of obese women with type 2 diabetes. Pediatr Res 82:768-775
Lucey, Brendan P; Mawuenyega, Kwasi G; Patterson, Bruce W et al. (2017) Associations Between ?-Amyloid Kinetics and the ?-Amyloid Diurnal Pattern in the Central Nervous System. JAMA Neurol 74:207-215
Hölttä, Mikko; Dean, Robert A; Siemers, Eric et al. (2016) A single dose of the ?-secretase inhibitor semagacestat alters the cerebrospinal fluid peptidome in humans. Alzheimers Res Ther 8:11
Karner, Courtney M; Esen, Emel; Chen, Jiakun et al. (2016) Wnt Protein Signaling Reduces Nuclear Acetyl-CoA Levels to Suppress Gene Expression during Osteoblast Differentiation. J Biol Chem 291:13028-39
Alvarez, Jessica A; Ziegler, Thomas R; Millson, Erin C et al. (2016) Body composition and lung function in cystic fibrosis and their association with adiposity and normal-weight obesity. Nutrition 32:447-52
Sterl, Karin; Wang, Songyan; Oestricker, Lauren et al. (2016) Metabolic responses to xenin-25 are altered in humans with Roux-en-Y gastric bypass surgery. Peptides 82:76-84

Showing the most recent 10 out of 696 publications