The major focus of our research program is to understand how cholesterol metabolism is regulated in higher animals. This is a significant goal since large variations can occur in both dietary intake and metabolic demand for this essential cellular metabolite. The regulation of cholesterol metabolism is achieved through feedback regulation of the expression of key proteins involved in its cellular uptake and biosynthesis. A major site for regulating cholesterol uptake and biosynthesis is at the level of transcription for genes that encode important proteins of both processes. The experiments will focus on special aspects of the activity and regulation of the SREBP proteins . These studies will involve 1) examing the potential interaction between SREBP-1 and-2, 2) monitor the time course of the sterol regulated processing of SREBP-1 and -2 from the ER membrane, and 3) evaluate whether the protein that regulates SREBP cleavage in the ER called SCAP co-localizes with either or both SREBP in the ER membrane and determine whether the proximity of SCAP to each SREBP changes during the process of sterol mediated regulation of its processing.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001192-20
Application #
6119304
Study Section
Project Start
1999-04-01
Project End
2000-03-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
20
Fiscal Year
1999
Total Cost
Indirect Cost
Name
University of California Irvine
Department
Type
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697
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