This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Sulfur is an essential element for plants, being converted into cysteine and then to other sulfur metabolites. Current research on sulfur metabolism in plants is severely restricted by the present 'wet chemistry' methods for identifying sulfur species, which require digestion/heating/destruction of the sample and only provide rather inaccurate 'total organic' vs. 'total inorganic' amounts of sulfur. By means of sulfur K-edge X-ray absorption spectroscopy (XAS) we will perform detailed speciation analyses of the major sulfur species present in intact plant leaves to clarify the key steps in the sulfur metabolism. We will also study samples from areas in Alberta, Canada, exposed to well-documented extensive sour gas emissions, to investigate the effects on the sulfur metabolism. Lichens, which only take up sulfur from the air, are great natural monitors for sour gas effects. Cysteine and its derivatives are medically used for heavy metal detoxification. Cysteine, one of 21 amino acids found in living organisms, contains three coordinating sites: the amino, thiol and carboxylate groups. The thiol group can form strong bonds and accumulate ions of soft, heavy metals. Coordination chemical studies have so far been focused on metal complexes in the solid state, mostly by elemental analysis, IR and in a few cases, X-ray crystallography. Little is known about the structure and coordination in solution, near to physiological conditions. We will use metal and sulfur XAS to study the local structure around the metal center, i.e. the metal-ligand bond distances, number and type of coordinating atoms, as well as the electronic environment around the sulfur atom in complexes of heavy metals, e.g. Pb, Cd, Hg and Ag, with cysteine and its derivatives.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-27
Application #
7370488
Study Section
Special Emphasis Panel (ZRG1-BPC-E (40))
Project Start
2006-03-01
Project End
2007-02-28
Budget Start
2006-03-01
Budget End
2007-02-28
Support Year
27
Fiscal Year
2006
Total Cost
$8,133
Indirect Cost
Name
Stanford University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
Vickers, Chelsea; Liu, Feng; Abe, Kento et al. (2018) Endo-fucoidan hydrolases from glycoside hydrolase family 107 (GH107) display structural and mechanistic similarities to ?-l-fucosidases from GH29. J Biol Chem 293:18296-18308
Nguyen, Phong T; Lai, Jeffrey Y; Lee, Allen T et al. (2018) Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter. Proc Natl Acad Sci U S A 115:E10596-E10604
Aleman, Fernando; Tzarum, Netanel; Kong, Leopold et al. (2018) Immunogenetic and structural analysis of a class of HCV broadly neutralizing antibodies and their precursors. Proc Natl Acad Sci U S A 115:7569-7574
Herrera, Nadia; Maksaev, Grigory; Haswell, Elizabeth S et al. (2018) Elucidating a role for the cytoplasmic domain in the Mycobacterium tuberculosis mechanosensitive channel of large conductance. Sci Rep 8:14566
Lal, Neeraj K; Nagalakshmi, Ugrappa; Hurlburt, Nicholas K et al. (2018) The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe 23:485-497.e5
Pluvinage, Benjamin; Grondin, Julie M; Amundsen, Carolyn et al. (2018) Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont. Nat Commun 9:1043
Beyerlein, Kenneth R; J├Ânsson, H Olof; Alonso-Mori, Roberto et al. (2018) Ultrafast nonthermal heating of water initiated by an X-ray Free-Electron Laser. Proc Natl Acad Sci U S A 115:5652-5657
Yoshizawa, Takuya; Ali, Rustam; Jiou, Jenny et al. (2018) Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites. Cell 173:693-705.e22
Guillaume, Joren; Wang, Jing; Janssens, Jonas et al. (2017) Galactosylsphingamides: new ?-GalCer analogues to probe the F'-pocket of CD1d. Sci Rep 7:4276
Ishigami, Izumi; Zatsepin, Nadia A; Hikita, Masahide et al. (2017) Crystal structure of CO-bound cytochrome c oxidase determined by serial femtosecond X-ray crystallography at room temperature. Proc Natl Acad Sci U S A 114:8011-8016

Showing the most recent 10 out of 604 publications