Abstract

A new NIH Resource is proposed in multi nuclei nuclear magnetic resonance (nmr) spectroscopy and spectral data processing. The goal of this Resource will be to develop and make available new instrumental and computer methods for structure/function analysis of biopolymers and complex biosystems. Specific objectives include: (1) development of probes for optimized high sensitivity 13C and other nuclei nmr of biological samples; (2) implementation of a new spectral data processing package having unique capabilities for utilization and presentation of raw spectroscopic data; (3) design of a hardware/software system to enable users remote to the Resource to have on-line access to the Resource computer and spectrometers, while experiments are in progress (these users will have local graphics and digital plotting capabilities); (4) design of a powerful data processing center based on the Resource 32-bit midicomputer and allowing database searching activity """"""""number crunching"""""""", as well as computer-assisted communication with Resource users.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001317-06
Application #
3103853
Study Section
(SSS)
Project Start
1981-08-01
Project End
1990-07-31
Budget Start
1986-08-01
Budget End
1987-07-31
Support Year
6
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Syracuse University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Syracuse
State
NY
Country
United States
Zip Code
13210

  Publications

LaPlante, S R; Zanatta, N; Hakkinen, A et al. (1994) 13C-NMR of the deoxyribose sugars in four DNA oligonucleotide duplexes: assignment and structural features. Biochemistry 33:2430-40
Borer, P N; LaPlante, S R; Kumar, A et al. (1994) 13C-NMR relaxation in three DNA oligonucleotide duplexes: model-free analysis of internal and overall motion. Biochemistry 33:2441-50
Kovacs, T; Parkanyi, L; Pelczer, I et al. (1991) Solid-state and solution conformation of 3'-amino-3'-deoxythymidine, precursor to a noncompetitive inhibitor of HIV-1 reverse transcriptase. J Med Chem 34:2595-600
Mazzeo, A R; Levy, G C (1991) An evaluation of new processing protocols for in vivo NMR spectroscopy. Magn Reson Med 17:483-95
Wang, Y Y; Lyttle, M H; Borer, P N (1990) Enzymatic and NMR analysis of oligoribonucleotides synthesized with 2'-tert-butyldimethylsilyl protected cyanoethylphosphoramidite monomers. Nucleic Acids Res 18:3347-52
Hyman, T J; Kurland, R J; Levy, G C et al. (1989) Characterization of normal brain tissue using seven calculated MRI parameters and a statistical analysis system. Magn Reson Med 11:22-34
Ni, F; Konishi, Y; Bullock, L D et al. (1989) High-resolution NMR studies of fibrinogen-like peptides in solution: structural basis for the bleeding disorder caused by a single mutation of Gly(12) to Val(12) in the A alpha chain of human fibrinogen Rouen. Biochemistry 28:3106-19
Glasel, J A (1989) Nuclear magnetic resonance studies of flexible opiate conformations at monoclonal antibody binding sites. Quantitative interproton distances obtained from comparing theoretical and experimental transferred nuclear Overhauser enhancement: correlation with J Mol Biol 209:747-61
Brainard, J R; Hutson, J Y; Hoekenga, D E et al. (1989) Ordered synthesis and mobilization of glycogen in the perfused heart. Biochemistry 28:9766-72
Grahn, H; Edlund, U; van den Hoogen, Y T et al. (1989) Toward a computer assisted analysis of NOESY spectra: a multivariate data analysis of an RNA NOESY spectrum. J Biomol Struct Dyn 6:1135-50

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