We propose a center devoted to research and development on software and hardware for use in analyzing the images obtained by modern neuroanatomical techniques, particularly the technique of 2-deoxglucose autoradiography, which marks neural systems on the basis of their metabolism and hence reveals alterations in their functional activity. The center will be lodged in an applications oriented computer engineering environment and will provide for collaboration between neuroscientists and computer engineers. It will also provide image processing as a service to a large number of major and minor users in the Northeast Corridor and throughout the country. The image analyzing routines will focus on the detection, quantification, and mapping of the experimental effects seen in autoradiographs and on the accurate collation of different images (e.g., autoradiographic and histological images). It will also foster the study of the reproducibility and validity of various kinds of quantification, particularly those involving some kind of normalization. It will develop low cost systems for performing the basic image analyzing functions needed by investigators using the 2DG technique. By means of collaborative projects, it will seek to make available algorithms for the dimensional reconstructions of neural systems both macro and micro, to extend the image analyzing algorithms to use with other modern neuronanastomical techniques (HRP, histofluorescence, and tritiated amino acids), and to tackle the image analyzing problems that arise in 2-dimensional gel electrophoresis.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR001638-03S2
Application #
3103952
Study Section
(SSS)
Project Start
1983-09-30
Project End
1988-06-30
Budget Start
1987-09-30
Budget End
1988-06-30
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Drexel University
Department
Type
DUNS #
061197161
City
Philadelphia
State
PA
Country
United States
Zip Code
19104
Ivey, Richard G; Subramanian, Oby; Lorentzen, Travis D et al. (2009) Antibody-based screen for ionizing radiation-dependent changes in the Mammalian proteome for use in biodosimetry. Radiat Res 171:549-61
Palha, J A; Nissanov, J; Fernandes, R et al. (2002) Thyroid hormone distribution in the mouse brain: the role of transthyretin. Neuroscience 113:837-47
Shumsky, Jed S; Wu, Yunxing; Murphy, E Hazel et al. (2002) Differential effects of prenatal cocaine exposure on selected subunit mRNAs of the GABA(A) receptor in rabbit anterior cingulate cortex. J Chem Neuroanat 24:243-55
Nissanov, J; Bertrand, L; Tretiak, O (2001) Cryosectioning distortion reduction using tape support. Microsc Res Tech 53:239-40
Komisaruk, B R; Rosenblatt, J S; Barona, M L et al. (2000) Combined c-fos and 14C-2-deoxyglucose method to differentiate site-specific excitation from disinhibition: analysis of maternal behavior in the rat. Brain Res 859:262-72
Del Cerro, M C (1998) Role of the vomeronasal input in maternal behavior. Psychoneuroendocrinology 23:905-26
Cohen, F S; Yang, Z; Huang, Z et al. (1998) Automatic matching of homologous histological sections. IEEE Trans Biomed Eng 45:642-9
Ali, W S; Cohen, F S (1998) Registering coronal histological 2-D sections of a rat brain with coronal sections of a 3-D brain atlas using geometric curve invariants and B-spline representation. IEEE Trans Med Imaging 17:957-66
Shumsky, J S; Wu, Y; Murphy, E H et al. (1998) Prenatal cocaine exposure does not affect selected GABAA receptor subunit mRNA expression in rabbit visual cortex. Ann N Y Acad Sci 846:371-4
Libon, D J; Bogdanoff, B; Cloud, B S et al. (1998) Declarative and procedural learning, quantitative measures of the hippocampus, and subcortical white alterations in Alzheimer's disease and ischaemic vascular dementia. J Clin Exp Neuropsychol 20:30-41

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