Martin Guthold joined the project December 1, 1997 to work with external biologists using the nanoManipulator in their investigations. He will also be continuing his investigations of DNA. We have initiated an investigation of DNA and protein-DNA complexes using the nanoManipulator and complementary biochemical studies. High-resolution images of DNA in air can now be obtained routinely. In initial pushing experiments of DNA adsorbed on mica in air, we were able to rupture DNA in a controlled matter. After proper calibration of the lateral force constant of the cantilever we will determine the rupture force of DNA. Experiments to determine the frictional force, the friction coefficient and the activation energy to move DNA on the surface are currently pursued. We have determined the binding energy of a 25 bp DNA fragment for adsorption onto mica for one set of conditions. The free energy of adsorption for this fragment was determined to be about 4.2 kT, the entropic energy loss was calculated to be about 1 kT, and the enthalpy of binding was, therefore, about 3.2 kT. Binding experiments will be done for different buffer and substrate conditions and for DNA fragments of different length. We are developing a theoretical framework to describe the adsorption of DNA to a substrate. Using a simple model, we have derived formulas for the length dependence of the free energy, enthalpy and entropy of adsorption. A complete model including the aforementioned parameters plus frictional parameters and the activation energy for motion on the surface will be developed in the future.
| Wu, Henry C; Yamankurt, Gokay; Luo, JiaLie et al. (2015) Identification and characterization of two ankyrin-B isoforms in mammalian heart. Cardiovasc Res 107:466-77 |
| Ahmed, Suzanne; Wang, Wei; Mair, Lamar O et al. (2013) Steering acoustically propelled nanowire motors toward cells in a biologically compatible environment using magnetic fields. Langmuir 29:16113-8 |
| Schafer, J; Foest, R; Reuter, S et al. (2012) Laser schlieren deflectometry for temperature analysis of filamentary non-thermal atmospheric pressure plasma. Rev Sci Instrum 83:103506 |
| Phadke, Madhura N; Pinto, Lifford; Alabi, Femi et al. (2012) Exploring Ensemble Visualization. Proc SPIE Int Soc Opt Eng 8294: |
| Caplan, Jeffrey; Niethammer, Marc; Taylor 2nd, Russell M et al. (2011) The power of correlative microscopy: multi-modal, multi-scale, multi-dimensional. Curr Opin Struct Biol 21:686-93 |
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| Mandal, Sudeep; Serey, Xavier; Erickson, David (2010) Nanomanipulation using silicon photonic crystal resonators. Nano Lett 10:99-104 |
| Feng, David; Kwock, Lester; Lee, Yueh et al. (2010) Matching visual saliency to confidence in plots of uncertain data. IEEE Trans Vis Comput Graph 16:980-9 |
| Quammen, Cory; Taylor 2nd, Russell M (2010) Adapting the ITK Registration Framework to Fit Parametric Image Models. Insight J :1-8 |
| Lai, Bonnie E; Geonnotti, Anthony R; Desoto, Michael G et al. (2010) Semi-solid gels function as physical barriers to human immunodeficiency virus transport in vitro. Antiviral Res 88:143-51 |
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