We propose to continue the operation of a national stable isotope resource (SIR) at the Los Alamos National Laboratory. The principal goal of the SIR is to promote new applications for the isotopes of carbon (12C, 13C0, oxygen (12O, 17O, 18O), nitrogen (14N, 15N), sulfur (32S, 33S, 34S, 36S), and to a lesser degree selenium (77Se) in solving fundamental problems in biochemistry, biophysics, as well as practical problems in medical science. The research component of the proposal suggests development of efficient procedures for specific labeling of amino acids, nucleic acids, enzyme cofactors, and new starting materials for syntheses. By making these materials available to the research community, the Resource will contribute to fundamental studies of protein and nucleic acid structure, protein-nucleic acid interactions, and structure-function relations in heme proteins. The Resource will continue to carry out a spectrum of core, service, collaborative, and training activities aimed at broadening the uses for stable isotopes in biomedical research. It will continue its contribution to the developing commerce in stable isotopically labeled compounds by devising efficient procedures for converting labeled CO and NO to useful materials. Particular compounds that become the focus of Resource activities will be selected in consultation with the Resource's Advisory Committee and will be provided to external investigators on a competitive basis. The latter is one of the most important services of the Resource.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR002231-09
Application #
3104074
Study Section
Special Emphasis Panel (SSS (R8))
Project Start
1983-12-01
Project End
1995-11-30
Budget Start
1992-01-15
Budget End
1992-11-30
Support Year
9
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Los Alamos National Lab
Department
Type
Organized Research Units
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
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Shiflett, Patrick R; Taylor-McCabe, Kirsten J; Michalczyk, Ryszard et al. (2003) Structural studies on the hairpins at the 3' untranslated region of an anthrax toxin gene. Biochemistry 42:6078-89
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