This is a proposal to continue the support of a Resource center for developing cryo-electron microscopy (cryo-EM) and image analysis technology. The ultimate goal of this Resource is 3-dimensional structure determination of biological macromolecules to near atomic resolution. The activities of our Resource include technological development, core and collaborative research, service, training, technology dissemination and maintaining an advisory committee. The technological development will be focused on the improvement of our JEOL4000 intermediate voltage electron microscope to make it an operationally efficient instrument for high resolution data collection at low temperature and low dose conditions. Experimental and computational strategies will be developed to determine 3- dimensional structures in four core research projects: crotoxin complex crystal, T4 gp32*I crystal, actin bundle from Limulus sperm and human herpes simplex virus. Technically, these specimens provide opportunities for different kinds of methodology development for thin crystals, in vivo crystalline arrays and large icosahedral spherical viruses. In addition, structural information from these specimens is relevant to our basic understanding of neurotoxicity, gene regulation, cytoskeleton biology and virology. An in-house expertise on cryo-sectioning procedure will be developed that will be useful for our study of the herpes viral genome structure. All these developed procedures and facilities will be used to investigate various biologically and medically important structures in collaboration with other researchers. In this proposal, we identify twelve such probable collaborative projects. Workshops or symposia will be held annually to disseminate our Resource technology and our staff members will be available to assist outside users in cryo-EM, EM of thick sectioned materials and image processing. A group of specialists will be assembled to meet annually to evaluate the progress and directions of the Resource activities.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002250-08
Application #
3104090
Study Section
Special Emphasis Panel (SSS (C))
Project Start
1988-09-01
Project End
1994-11-30
Budget Start
1992-12-01
Budget End
1993-11-30
Support Year
8
Fiscal Year
1993
Total Cost
Indirect Cost
Name
Baylor College of Medicine
Department
Type
Schools of Medicine
DUNS #
074615394
City
Houston
State
TX
Country
United States
Zip Code
77030
Bucero, Marta Abril; Bajaj, Chandrajit; Mourrain, Bernard (2016) On the construction of general cubature formula by flat extensions. Linear Algebra Appl 502:104-125
Ebeida, Mohamed S; Rushdi, Ahmad A; Awad, Muhammad A et al. (2016) Disk Density Tuning of a Maximal Random Packing. Comput Graph Forum 35:259-269
Wensel, Theodore G; Zhang, Zhixian; Anastassov, Ivan A et al. (2016) Structural and molecular bases of rod photoreceptor morphogenesis and disease. Prog Retin Eye Res 55:32-51
Jeter, Cameron B; Patel, Saumil S; Morris, Jeffrey S et al. (2015) Oculomotor executive function abnormalities with increased tic severity in Tourette syndrome. J Child Psychol Psychiatry 56:193-202
Zhang, Qin; Cha, Deukhyun; Bajaj, Chandrajit (2015) Quality Partitioned Meshing of Multi-Material Objects. Procedia Eng 124:187-199
Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-particle cryo-EM of the ryanodine receptor channel in an aqueous environment. Eur J Transl Myol 25:35-48
Bettadapura, Radhakrishna; Rasheed, Muhibur; Vollrath, Antje et al. (2015) PF2fit: Polar Fast Fourier Matched Alignment of Atomistic Structures with 3D Electron Microscopy Maps. PLoS Comput Biol 11:e1004289
Baranovskiy, Andrey G; Zhang, Yinbo; Suwa, Yoshiaki et al. (2015) Crystal structure of the human primase. J Biol Chem 290:5635-46
Zhang, Zhixian; He, Feng; Constantine, Ryan et al. (2015) Domain organization and conformational plasticity of the G protein effector, PDE6. J Biol Chem 290:12833-43
Baker, Mariah R; Fan, Guizhen; Serysheva, Irina I (2015) Single-Particle Cryo-EM of the Ryanodine Receptor Channel in an Aqueous Environment. Eur J Transl Myol 25:4803

Showing the most recent 10 out of 213 publications