Agency
National Institute of Health (NIH)
Institute
National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)
Type
Research Project (R01)
Project #
2R01AR017323-24
Application #
2078361
Study Section
Bio-Organic and Natural Products Chemistry Study Section (BNP)
Project Start
1978-05-01
Project End
2000-04-30
Budget Start
1996-05-01
Budget End
1997-04-30
Support Year
24
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of California San Francisco
Department
Pharmacology
Type
Schools of Pharmacy
DUNS #
073133571
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Novak, Walter R P; Wang, Pan-Fen; McLeish, Michael J et al. (2004) Isoleucine 69 and valine 325 form a specificity pocket in human muscle creatine kinase. Biochemistry 43:13766-74
Lahiri, Sushmita D; Wang, Pan-Fen; Babbitt, Patricia C et al. (2002) The 2.1 A structure of Torpedo californica creatine kinase complexed with the ADP-Mg(2+)-NO(3)(-)-creatine transition-state analogue complex. Biochemistry 41:13861-7
Wang, Pan-Fen; Novak, Walter R P; Cantwell, John S et al. (2002) Expression of Torpedo californica creatine kinase in Escherichia coli and purification from inclusion bodies. Protein Expr Purif 26:89-95
Wang, P F; McLeish, M J; Kneen, M M et al. (2001) An unusually low pK(a) for Cys282 in the active site of human muscle creatine kinase. Biochemistry 40:11698-705
Cantwell, J S; Novak, W R; Wang, P F et al. (2001) Mutagenesis of two acidic active site residues in human muscle creatine kinase: implications for the catalytic mechanism. Biochemistry 40:3056-61
Huang, C C; Novak, W R; Babbitt, P C et al. (2000) Integrated tools for structural and sequence alignment and analysis. Pac Symp Biocomput :230-41
Chen, L H; White, C B; Babbitt, P C et al. (2000) A comparative study of human muscle and brain creatine kinases expressed in Escherichia coli. J Protein Chem 19:59-66
Wood, T D; Guan, Z; Borders Jr, C L et al. (1998) Creatine kinase: essential arginine residues at the nucleotide binding site identified by chemical modification and high-resolution tandem mass spectrometry. Proc Natl Acad Sci U S A 95:3362-5
Chen, L H; Borders Jr, C L; Vasquez, J R et al. (1996) Rabbit muscle creatine kinase: consequences of the mutagenesis of conserved histidine residues. Biochemistry 35:7895-902
Wood, T D; Chen, L H; Kelleher, N L et al. (1995) Direct sequence data from heterogeneous creatine kinase (43 kDa) by high-resolution tandem mass spectrometry. Biochemistry 34:16251-4

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