Abstract

We have recently developed a new method for indirect detection of 17O. This method uses scalar coupling between 1H and 17O in H217O. Scalar coupling between 17O, a spin 5/2 nucleus, and 1H in 1H217O is about 92 Hz. At neutral pH, protons in H217O exchange with that in H216O. This exchange and the T1 processes of 17O modulate the scalar coupling. This modulated scalar coupling causes proton line broadening in H217O. The present method exploits the properties of 1H217O in the presence of a spin-locking radiofrequency field. We have demonstrated the strong dependence of T1r relaxation time of H217O on the strength of spin-locking field. This dependence of T1r on the strength of spin-locking field is known as T1r dispersion of the sample. This method has potential application in the study of oxidative phosphorylation and blood flow. We have implemented this method in vivo on mouse brain. Mouse brain was imaged with T1r sequence following injection of known amount of H217O. The ratio image obtained from two T1r images has been used to calculate the image intensity. The 17O concentration calculated is in excellent agreement with that of actual. Further in vivo studies are in progress.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR002305-13
Application #
5224003
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
13
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Pang, Henry; Bow, Cora; Cheung, Jason Pui Yin et al. (2018) The UTE Disc Sign on MRI: A Novel Imaging Biomarker Associated With Degenerative Spine Changes, Low Back Pain, and Disability. Spine (Phila Pa 1976) 43:503-511
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Yadav, Santosh K; Kathiresan, Nagarajan; Mohan, Suyash et al. (2016) Gender-based analysis of cortical thickness and structural connectivity in Parkinson's disease. J Neurol 263:2308-2318
Finkelstein, Joel S; Lee, Hang; Leder, Benjamin Z et al. (2016) Gonadal steroid-dependent effects on bone turnover and bone mineral density in men. J Clin Invest 126:1114-25
Rosenbaum, Michael; Leibel, Rudolph L (2016) Models of energy homeostasis in response to maintenance of reduced body weight. Obesity (Silver Spring) 24:1620-9
Machida, Manabu; Panasyuk, George Y; Wang, Zheng-Min et al. (2016) Radiative transport and optical tomography with large datasets. J Opt Soc Am A Opt Image Sci Vis 33:551-8
Ban, H Y; Schweiger, M; Kavuri, V C et al. (2016) Heterodyne frequency-domain multispectral diffuse optical tomography of breast cancer in the parallel-plane transmission geometry. Med Phys 43:4383
McCarthy, Ann L; Winters, Madeline E; Busch, David R et al. (2015) Scoring system for periventricular leukomalacia in infants with congenital heart disease. Pediatr Res 78:304-9

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