Abstract

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. We are using trimodal spectroscopy for the detection of dysplasia and cancer in vivo in the uterine cervix. We have developed the FastEEM spectrofluorometer to collect reflectance and fluorescence spectra in vivo via a fiber optic probe. Spectra are analyzed using trimodal spectroscopic algorithms (i.e. the combination of three spectroscopic techniques: diffuse reflectance, light scattering, and intrinsic fluorescence spectroscopy) to obtain biochemical, structural, and morphological information about the tissue. Tissue is classified as normal squamous, normal columnar, mataplastic, low-grade, or high-grade dysplasia. We are developing real-time diagnostic algorithms so that the FastEEM spectrofluorometer can be used as a guide to biopsy.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR002594-21
Application #
7357925
Study Section
Special Emphasis Panel (ZRG1-SBIB-L (40))
Project Start
2006-06-01
Project End
2007-05-31
Budget Start
2006-06-01
Budget End
2007-05-31
Support Year
21
Fiscal Year
2006
Total Cost
$20,347
Indirect Cost

  Institution

Name
Massachusetts Institute of Technology
Department
Internal Medicine/Medicine
Type
Schools of Arts and Sciences
DUNS #
001425594
City
Cambridge
State
MA
Country
United States
Zip Code
02139

  Publications

Shih, Wei-Chuan; Bechtel, Kate L; Rebec, Mihailo V (2015) Noninvasive glucose sensing by transcutaneous Raman spectroscopy. J Biomed Opt 20:051036
Dudzik, Jonathan; Chang, Wen-Chi; Kannan, A M et al. (2013) Cross-linked glucose oxidase clusters for biofuel cell anode catalysts. Biofabrication 5:035009
Sathyavathi, R; Dingari, Narahara Chari; Barman, Ishan et al. (2013) Raman spectroscopy provides a powerful, rapid diagnostic tool for the detection of tuberculous meningitis in ex vivo cerebrospinal fluid samples. J Biophotonics 6:567-72
Dingari, Narahara Chari; Barman, Ishan; Saha, Anushree et al. (2013) Development and comparative assessment of Raman spectroscopic classification algorithms for lesion discrimination in stereotactic breast biopsies with microcalcifications. J Biophotonics 6:371-81
Cooper, Kimberly L; Oh, Seungeun; Sung, Yongjin et al. (2013) Multiple phases of chondrocyte enlargement underlie differences in skeletal proportions. Nature 495:375-8
Sung, Yongjin; Tzur, Amit; Oh, Seungeun et al. (2013) Size homeostasis in adherent cells studied by synthetic phase microscopy. Proc Natl Acad Sci U S A 110:16687-92
Lau, Condon; Mirkovic, Jelena; Yu, Chung-Chieh et al. (2013) Early detection of high-grade squamous intraepithelial lesions in the cervix with quantitative spectroscopic imaging. J Biomed Opt 18:76013
Soares, Jaqueline S; Barman, Ishan; Dingari, Narahara Chari et al. (2013) Diagnostic power of diffuse reflectance spectroscopy for targeted detection of breast lesions with microcalcifications. Proc Natl Acad Sci U S A 110:471-6
Barman, Ishan; Dingari, Narahara Chari; Kang, Jeon Woong et al. (2012) Raman spectroscopy-based sensitive and specific detection of glycated hemoglobin. Anal Chem 84:2474-82
Kalashnikov, Maxim; Choi, Wonshik; Hunter, Martin et al. (2012) Assessing the contribution of cell body and intracellular organelles to the backward light scattering. Opt Express 20:816-26

Showing the most recent 10 out of 178 publications