Abstract

Our studies were conducted to determine (1) the effects of cholesterol on renal proximal tubular apical brush border membrane fluidity and ion transport activity, both in renal tubular cells grown in culture, and in isolated brush border membranes; (2) the role of alterations in membrane cholesterol and phospholipid composition and membrane fluidity in adaptation of renal tubular cell phosphate transporter to acute and chronic perturbations in extracellular phosphate concentration; and (3) the role of alterations in membrane lipid composition and fluidity in the response of the renal phosphate transport to extracellular hormones. Consulting: 36 hours with Weiming Yu to help set-up new 2-photon computer and Fluorescence microscope, on site. Also installed computer hardware and software for microscope

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR003155-15
Application #
6348041
Study Section
Project Start
2000-08-01
Project End
2001-07-31
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
15
Fiscal Year
2000
Total Cost
$680
Indirect Cost

  Institution

Name
University of Illinois Urbana-Champaign
Department
Type
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820

  Publications

Kim, Seong M; Nguyen, Tricia T; Ravi, Archna et al. (2018) PTEN Deficiency and AMPK Activation Promote Nutrient Scavenging and Anabolism in Prostate Cancer Cells. Cancer Discov 8:866-883
Liang, Elena I; Mah, Emma J; Yee, Albert F et al. (2017) Correlation of focal adhesion assembly and disassembly with cell migration on nanotopography. Integr Biol (Camb) 9:145-155
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2016) Self-assisted optothermal trapping of gold nanorods under two-photon excitation. Methods Appl Fluoresc 4:035003
Digiacomo, Luca; Digman, Michelle A; Gratton, Enrico et al. (2016) Development of an image Mean Square Displacement (iMSD)-based method as a novel approach to study the intracellular trafficking of nanoparticles. Acta Biomater 42:189-198
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Chen, Hongtao; Gratton, Enrico; Digman, Michelle A (2015) Spectral properties and dynamics of gold nanorods revealed by EMCCD-based spectral phasor method. Microsc Res Tech 78:283-93
Golfetto, Ottavia; Hinde, Elizabeth; Gratton, Enrico (2015) The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells. Methods Mol Biol 1232:273-90
Willenberg, Rafer; Steward, Oswald (2015) Nonspecific labeling limits the utility of Cre-Lox bred CST-YFP mice for studies of corticospinal tract regeneration. J Comp Neurol 523:2665-82
Bonaventura, Gabriele; Barcellona, Maria Luisa; Golfetto, Ottavia et al. (2014) Laurdan monitors different lipids content in eukaryotic membrane during embryonic neural development. Cell Biochem Biophys 70:785-94
Paladino, Simona; Lebreton, Stéphanie; Tivodar, Simona et al. (2014) Golgi sorting regulates organization and activity of GPI proteins at apical membranes. Nat Chem Biol 10:350-357

Showing the most recent 10 out of 200 publications