A general solution to the problem of localizing nuclear magneticresonance spectra for biomedical research and clinicalapplications is being extended and perfected. The method, spectrallocalization by imaging (SLIM) and its generalized extension(GSLIM), can use anatomical information, as obtained routinely bystandard MRI methods, to define any number of volume elements ofany shape. This information may be combined mathematically withspectroscopic data, yielding metabolic concentrations in allanatomical regions simultaneously, but in a much shorter time thanrequired for conventional chemical shift imaging. GSLIM alsoreveals concentration or other variations within regions. RESEARCH HIGHLIGHTS:Thorough theoretical analyses and computer simulations of themethod have been carried out. Our recent results confirm that SLIMand GSLIM are reliable and efficient methods for measuring averagemetabolic concentrations from volume elements of any shape underpractical conditions (noise and inhomogeneities of any kind insidethe volume elements). These results have been further verified bya new complex-phantom study. We have also applied the method toobtain localized 31P from models derived from human images. We areable to derive good quality localized spectra from regions ofcerebellum, cerebrum, gray and white matter. We have now usedthese methods for quantitative analysis, and carried out furtherextensive verification studies.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Li, Li; Palencia, Andrés; Lukk, Tiit et al. (2013) Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens. Proc Natl Acad Sci U S A 110:3817-22
Li, Li; Boniecki, Michal T; Jaffe, Jacob D et al. (2011) Naturally occurring aminoacyl-tRNA synthetases editing-domain mutations that cause mistranslation in Mycoplasma parasites. Proc Natl Acad Sci U S A 108:9378-83
Sallam, Khalid Ibrahim (2007) Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control 18:566-575
Sallam, Khalid Ibrahim (2007) Chemical, sensory and shelf life evaluation of sliced salmon treated with salts of organic acids. Food Chem 101:592-600
Sallam, Kh I; Samejima, K (2004) Microbiological and chemical quality of ground beef treated with sodium lactate and sodium chloride during refrigerated storage. Lebenson Wiss Technol 37:865-871
Moser, Kevin W; Georgiadis, John G (2004) Extraction and validation of correlation lengths from interstitial velocity fields using diffusion-weighted MRI. Magn Reson Imaging 22:257-68
Sallam, Kh I; Ishioroshi, M; Samejima, K (2004) Antioxidant and antimicrobial effects of garlic in chicken sausage. Lebenson Wiss Technol 37:849-855
Sallam, Khalid Ibrahim; Samejima, Kunihiko (2004) Effects of Trisodium Phosphate and Sodium Chloride Dipping on the Microbial Quality and Shelf Life of Refrigerated Tray-packaged Chicken Breasts. Food Sci Biotechnol 13:425-429
Wang, Steven J; Brechbiel, Martin; Wiener, Erik C (2003) Characteristics of a new MRI contrast agent prepared from polypropyleneimine dendrimers, generation 2. Invest Radiol 38:662-8
Swain, R A; Harris, A B; Wiener, E C et al. (2003) Prolonged exercise induces angiogenesis and increases cerebral blood volume in primary motor cortex of the rat. Neuroscience 117:1037-46

Showing the most recent 10 out of 55 publications