Abstract

The 1D 1H NMR experiments on Ribonuclease A protein suggests thatpressure denatured RNase A contains more partially foldedstructures than thermally denatured protein. In order tounderstand the details of the pressure denatured structure ofRNase A, we will do the amide hydrogen exchange experiment on theprotein. By comparing the fingerprint region of COSY spectrabefore and after pressurization, we will be able to tell whetherthe pressure denatured protein contains the elements of thesecondary structures or not.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR005964-06A1
Application #
5225194
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
6
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Li, Li; Palencia, Andrés; Lukk, Tiit et al. (2013) Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens. Proc Natl Acad Sci U S A 110:3817-22
Li, Li; Boniecki, Michal T; Jaffe, Jacob D et al. (2011) Naturally occurring aminoacyl-tRNA synthetases editing-domain mutations that cause mistranslation in Mycoplasma parasites. Proc Natl Acad Sci U S A 108:9378-83
Sallam, Khalid Ibrahim (2007) Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control 18:566-575
Sallam, Khalid Ibrahim (2007) Chemical, sensory and shelf life evaluation of sliced salmon treated with salts of organic acids. Food Chem 101:592-600
Sallam, Kh I; Samejima, K (2004) Microbiological and chemical quality of ground beef treated with sodium lactate and sodium chloride during refrigerated storage. Lebenson Wiss Technol 37:865-871
Moser, Kevin W; Georgiadis, John G (2004) Extraction and validation of correlation lengths from interstitial velocity fields using diffusion-weighted MRI. Magn Reson Imaging 22:257-68
Sallam, Kh I; Ishioroshi, M; Samejima, K (2004) Antioxidant and antimicrobial effects of garlic in chicken sausage. Lebenson Wiss Technol 37:849-855
Sallam, Khalid Ibrahim; Samejima, Kunihiko (2004) Effects of Trisodium Phosphate and Sodium Chloride Dipping on the Microbial Quality and Shelf Life of Refrigerated Tray-packaged Chicken Breasts. Food Sci Biotechnol 13:425-429
Wang, Steven J; Brechbiel, Martin; Wiener, Erik C (2003) Characteristics of a new MRI contrast agent prepared from polypropyleneimine dendrimers, generation 2. Invest Radiol 38:662-8
Swain, R A; Harris, A B; Wiener, E C et al. (2003) Prolonged exercise induces angiogenesis and increases cerebral blood volume in primary motor cortex of the rat. Neuroscience 117:1037-46

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