Abstract

31P and 1H spectra have been obtained on intact isolated and superfused toad retinae. Up to eight retinae are studied in a commercial probe and a single retina is studied in a purpose-built small-sample spectroscopy probe. Adequacy of tissue maintenance conditions has been demonstrated. Differences in phosphorus metabolite concentrations have been observed under conditions of light and dark adaptation. Cyclic GMP has been demonstrated in the 31P NMR spectrum of retina.The Pa/Pi ratio increases and [La] decreases in high calcium solution, a useful finding since calcium is thought to be involved in light adaptation.When the Na/K pump is blocked with ouabain in low calcium, both Pa/Pi and lactate decrease, suggesting differing functional roles for anaerobic and aerobic glycolysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR005964-10
Application #
6309679
Study Section
Project Start
2000-01-15
Project End
2002-01-14
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
10
Fiscal Year
2000
Total Cost
$23,005
Indirect Cost

  Institution

Name
University of Illinois at Chicago
Department
Type
DUNS #
098987217
City
Chicago
State
IL
Country
United States
Zip Code
60612

  Publications

Li, Li; Palencia, Andrés; Lukk, Tiit et al. (2013) Leucyl-tRNA synthetase editing domain functions as a molecular rheostat to control codon ambiguity in Mycoplasma pathogens. Proc Natl Acad Sci U S A 110:3817-22
Li, Li; Boniecki, Michal T; Jaffe, Jacob D et al. (2011) Naturally occurring aminoacyl-tRNA synthetases editing-domain mutations that cause mistranslation in Mycoplasma parasites. Proc Natl Acad Sci U S A 108:9378-83
Sallam, Khalid Ibrahim (2007) Antimicrobial and antioxidant effects of sodium acetate, sodium lactate, and sodium citrate in refrigerated sliced salmon. Food Control 18:566-575
Sallam, Khalid Ibrahim (2007) Chemical, sensory and shelf life evaluation of sliced salmon treated with salts of organic acids. Food Chem 101:592-600
Sallam, Kh I; Samejima, K (2004) Microbiological and chemical quality of ground beef treated with sodium lactate and sodium chloride during refrigerated storage. Lebenson Wiss Technol 37:865-871
Moser, Kevin W; Georgiadis, John G (2004) Extraction and validation of correlation lengths from interstitial velocity fields using diffusion-weighted MRI. Magn Reson Imaging 22:257-68
Sallam, Kh I; Ishioroshi, M; Samejima, K (2004) Antioxidant and antimicrobial effects of garlic in chicken sausage. Lebenson Wiss Technol 37:849-855
Sallam, Khalid Ibrahim; Samejima, Kunihiko (2004) Effects of Trisodium Phosphate and Sodium Chloride Dipping on the Microbial Quality and Shelf Life of Refrigerated Tray-packaged Chicken Breasts. Food Sci Biotechnol 13:425-429
Swain, R A; Harris, A B; Wiener, E C et al. (2003) Prolonged exercise induces angiogenesis and increases cerebral blood volume in primary motor cortex of the rat. Neuroscience 117:1037-46
Moser, Kevin W; Raguin, L Guy; Georgiadis, John G (2003) Synchronized EPI phase contrast velocimetry in a mixing reactor. Magn Reson Imaging 21:127-33

Showing the most recent 10 out of 55 publications