Although the ultimate targets of many signal transduction pathways are nuclear actors, the vast majority of known protein kinases are cytosolic. This project investigates a novel human kinase that is present exclusively in the nucleus. Kinase activity is increased upon cellular proliferation and is markedly elevated in patients with acute and lymphocytic leukemias. We have identified a human gene that encodes this nuclear kinase and find that it is closely related to Drosophilia female sterile homeotic (fsh), a developmental regulator with no known biological activity. Collectively, these results suggest that this nuclear kinase is a component of a signal transduction pathway that plays a role in Drosophilia development and human growth control. The posttranslational modifications of the protein are nowbeing investigated by mass spectrometry. Later, the association of this protein with others in itsactive complex will be probed.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR010888-03
Application #
6123253
Study Section
Project Start
1998-07-01
Project End
1999-06-30
Budget Start
Budget End
Support Year
3
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Boston University
Department
Type
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
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Steinhorn, Benjamin S; Loscalzo, Joseph; Michel, Thomas (2015) Nitroglycerin and Nitric Oxide--A Rondo of Themes in Cardiovascular Therapeutics. N Engl J Med 373:277-80

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